Issue 21, 2023

A reactive matrix for in situ chemical derivatisation and specific detection of cis-diol compounds by matrix-assisted laser desorption/ionisation mass spectrometry

Abstract

Analysis of cis-diol compounds is essential, because they play important roles in cosmetics, food, pharmaceuticals, and living organisms. Herein, we describe the development of a matrix-assisted laser desorption/ionisation mass spectrometry (MALDI-MS) method to analyse cis-diol compounds. In this method, a 6-borono-1-methylquinoline-1-ium (BMQI) reactive matrix was designed for in situ derivatisation of cis-diol compounds based on the boronate affinity interaction between boronic acid and cis-diol groups. Compared to traditional commercial matrices and other boronic acid reagents, BMQI can significantly accelerate the desorption/ionisation process, improve reproducibility, exhibit free background interference, and enhance signal intensity in the analysis of various cis-diol compounds even for amounts as low as 1 nmol. The BMQI-assisted laser desorption/ionisation mass spectrometry (LDI-MS) was successfully applied to the rapid screening and identification of sugar alcohols in different sugar-free foods. This work provides an alternative method to the LDI-MS analysis of cis-diol-containing molecules, and the method can be extended to other food samples and biofluids.

Graphical abstract: A reactive matrix for in situ chemical derivatisation and specific detection of cis-diol compounds by matrix-assisted laser desorption/ionisation mass spectrometry

Supplementary files

Article information

Article type
Paper
Submitted
16 aug 2023
Accepted
18 sep 2023
First published
18 sep 2023

Analyst, 2023,148, 5402-5406

A reactive matrix for in situ chemical derivatisation and specific detection of cis-diol compounds by matrix-assisted laser desorption/ionisation mass spectrometry

J. Chen, H. Huang, D. Ouyang, J. Lin, Z. Chen, Z. Cai and Z. Lin, Analyst, 2023, 148, 5402 DOI: 10.1039/D3AN01400B

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