Issue 6, 2023

Separation of amyloid β fragment peptides with racemised and isomerised aspartic acid residues using an original chiral resolution labeling reagent

Abstract

We developed a system to separate and identify racemised and isomerised aspartic acid (Asp) residues in amyloid β (Aβ) by labeling with an original chiral resolution labeling reagent, 1-fluoro-2,4-dinitrophenyl-5-D-leucine-N,N-dimethylethylenediamine-amide (D-FDLDA). The racemised and isomerised Asp residues labeled with D-FDLDA in Aβ fragments generated by digesting with trypsin and endoproteinase Glu-C were separated and identified by liquid chromatography–mass spectrometry (LC-MS) under simple gradient conditions. Furthermore, the labeled Aβ fragments did not aggregate and remained stable at least for 1 week at 4 °C.

Graphical abstract: Separation of amyloid β fragment peptides with racemised and isomerised aspartic acid residues using an original chiral resolution labeling reagent

Supplementary files

Article information

Article type
Communication
Submitted
18 nov 2022
Accepted
05 feb 2023
First published
06 feb 2023

Analyst, 2023,148, 1209-1213

Separation of amyloid β fragment peptides with racemised and isomerised aspartic acid residues using an original chiral resolution labeling reagent

M. Ozaki, M. Shimotsuma, T. Kuranaga, H. Kakeya and T. Hirose, Analyst, 2023, 148, 1209 DOI: 10.1039/D2AN01885C

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