Issue 13, 2019

Nanoscale organization of tetraspanins during HIV-1 budding by correlative dSTORM/AFM

Abstract

Membrane partition and remodeling play a key role in numerous cell mechanisms, especially in viral replication cycles where viruses subvert the plasma membrane to enter and escape from the host cell. Specifically assembly and release of HIV-1 particles require specific cellular components, which are recruited to the egress site by the viral protein Gag. We previously demonstrated that HIV-1 assembly alters both partitioning and dynamics of the tetraspanins CD9 and CD81, which are key players in many infectious processes, forming enriched areas where the virus buds. In this study we correlated super resolution microscopy mapping of tetraspanins with membrane topography delineated by atomic force microscopy (AFM) in Gag-expressing cells. We revealed that CD9 is specifically trapped within the nascent viral particles, especially at buds tips, suggesting that Gag mediates CD9 and CD81 depletion from the plasma membrane. In addition, we showed that CD9 is organized as small membrane assemblies of few tens of nanometers that can coalesce upon Gag expression.

Graphical abstract: Nanoscale organization of tetraspanins during HIV-1 budding by correlative dSTORM/AFM

Supplementary files

Article information

Article type
Paper
Submitted
06 sep 2018
Accepted
01 mar 2019
First published
07 mar 2019

Nanoscale, 2019,11, 6036-6044

Nanoscale organization of tetraspanins during HIV-1 budding by correlative dSTORM/AFM

S. Dahmane, C. Doucet, A. Le Gall, C. Chamontin, P. Dosset, F. Murcy, L. Fernandez, D. Salas, E. Rubinstein, M. Mougel, M. Nollmann and P. Milhiet, Nanoscale, 2019, 11, 6036 DOI: 10.1039/C8NR07269H

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements