Issue 11, 2019

Widefield frequency domain fluorescence lifetime imaging microscopy (FD-FLIM) for accurate measurement of oxygen gradients within microfluidic devices

Abstract

An oxygen gradient is a key variable influencing various biological activities in vivo, such as tissue repair and tumor growth. To study the phenomenon, in vitro cell studies using microfluidic devices capable of generating oxygen gradients have been developed recently. However, it is challenging to accurately measure the gradient profiles in devices. The traditional fluorescence intensity-based method suffers from the difficulty of accurate measurement due to background fluorescence artefacts. In addition, it is hard to obtain accurate calibration conditions because of the difficulties to achieve a fully depleted and saturated oxygen concentrations in the devices. To overcome these difficulties, a widefield frequency domain fluorescence imaging microscopy (FD-FLIM) system was constructed and utilized to accurately measure oxygen gradient profiles in a microfluidic device in this paper. Since lifetime-based measurements do not solely depend on intensity variations, oxygen calibration processes are amiable and the measured oxygen concentrations can be more accurate. The performance of the FD-FLIM system was validated by comparing the experimental and simulation results in microfluidic devices with different geometries. The experimental results show that the oxygen gradients generated from the chemical reaction method can provide more hypoxic oxygen conditions compared to the gradients created by the gas flowing method. Owing to the advantages provided by the widefield microscopy technique, the image acquisition time can be significantly reduced resulting in less photobleaching for time-lapsed imaging applications. Consequently, the measurement technique developed in this paper is an efficient tool, which can greatly help scientists to better study biological activities under various oxygen conditions.

Graphical abstract: Widefield frequency domain fluorescence lifetime imaging microscopy (FD-FLIM) for accurate measurement of oxygen gradients within microfluidic devices

Supplementary files

Article information

Article type
Paper
Submitted
22 jan 2019
Accepted
20 apr 2019
First published
22 apr 2019

Analyst, 2019,144, 3494-3504

Widefield frequency domain fluorescence lifetime imaging microscopy (FD-FLIM) for accurate measurement of oxygen gradients within microfluidic devices

H. Wu, T. Lee, P. Ko, W. Liao, T. Hsieh and Y. Tung, Analyst, 2019, 144, 3494 DOI: 10.1039/C9AN00143C

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements