Issue 21, 2015

Imaging of living cells and zebrafish in vivo using a ratiometric fluorescent probe for hydrogen sulfide

Abstract

We have developed a novel colorimetric and ratiometric fluorescence probe for the selective and sensitive monitoring of hydrogen sulfide based on a dicyanoisophorone platform. An excellent linear relationship of fluorescence intensity ratio (I637/I558) (R2 = 0.9867) versus hydrogen sulfide concentration in the range of 1–12 μM was obtained. This probe exhibited a remarkable fluorescence response to hydrogen sulfide over other physiological thiols or biological species, which fluoresces in the red region with a large Stokes shift (172 nm). This probe was successfully utilized to monitor H2S under in vitro physiological conditions and for imaging H2S in living cells and living zebrafish in vivo.

Graphical abstract: Imaging of living cells and zebrafish in vivo using a ratiometric fluorescent probe for hydrogen sulfide

Supplementary files

Article information

Article type
Communication
Submitted
19 jan 2015
Accepted
13 sep 2015
First published
15 sep 2015

Analyst, 2015,140, 7165-7169

Imaging of living cells and zebrafish in vivo using a ratiometric fluorescent probe for hydrogen sulfide

T. Liu, J. Lin, Z. Li, L. Lin, Y. Shen, H. Zhu and Y. Qian, Analyst, 2015, 140, 7165 DOI: 10.1039/C5AN00119F

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