CRISPR–Cas based platforms for RNA detection: fundamentals and applications

Mahsa Bagi, Sina Jamalzadegan, Anastasiia Steksova and Qingshan Wei

Chem. Commun., 2025, 61, 13571
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Rapid, multiplex, one-pot CRISPR/Dx system for detecting cancer fusion genes

Jiaqi Li, Cia-Hin Lau, Jianchao Wang, Weidong Wu, Zhihao Huang, Xiaoqing Chen, Jiahui Li, Yumei Huang, Tao Wang, Yulin Li, Zihan Zhao, Meijing Xu, Gang Chen, Sheng Tong and Haibao Zhu

Anal. Methods, 2025, 17, 6304
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A LEGO-inspired multipiece chip for portable nucleic acid detection

Ye Peng, Lu Gao, Pengkun Yin, Tianyu Dong, Ke Yi and Feng Li

Chem. Commun., 2026, 62, 5678
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Recent advances in the CRISPR/Cas system-based visual detection method

Jinrong Chen, Hang Su, June Hyun Kim, Lishang Liu and Rui Liu

Anal. Methods, 2024, 16, 6599
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Cascade signal amplification-based fluorescent biosensor utilizing exonuclease III and self-locking DNAzyme synergy for microRNA detection

Daqi Chen, Zhezhi Fang, Yachunyue Zhou, Ziyao Zeng, Jiancong Liang, Baian Zhu and Chaozhan Chen

Analyst, 2025, 150, 5023
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A high-fidelity DNAzyme-assisted CRISPR/Cas13a system with single-nucleotide resolved specificity

Yunping Wu, Ruigang Jin, Yangyang Chang and Meng Liu

Chem. Sci., 2024, 15, 6934
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CRISPR/Cas12a assay for amol level microRNA by combining enzyme-free amplification and single particle analysis

Chengchao Zhang, Xin Zhao, Xiao Chen, Xu Lin, Zili Huang, Jianyu Hu, Rui Liu and Yi Lv

Chem. Commun., 2024, 60, 13259
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Important applications of DNA nanotechnology combined with CRISPR/Cas systems in biotechnology

Yuqi Huang, Zhongping Chen, Huacui Huang, Shijia Ding and Mingjun Zhang

RSC Adv., 2025, 15, 6208
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RAA-CRISPR-HCR cascade amplification for ultrasensitive visual detection of African swine fever virus DNA

Hongyu Wu, Shuhan Lin, Xiangcun Zuo, Weishi Hua, Tao Sun, Guoqing Shao, Fang Li, Dongming Zhao, Zhixin Feng, Dan Zhu and Lianhui Wang

Chem. Commun., 2026, 62, 4329
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CRISPR/Cas12a platform activated by a protospacer adjacent motif-engineered DNA circuit for specific target sensing

Rui Wang, Jiayue Li, Yuchen Zheng, Ning Wei and Wei Li

Anal. Methods, 2026, 18, 1397
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Recent progress on the CRISPR/Cas system in optical biosensors

Bingqian Li, Guangyu Zhai, Yaru Dong, Lan Wang and Peng Ma

Anal. Methods, 2024, 16, 798
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CRISPR for companion diagnostics in low-resource settings

Xu Qian, Qiang Xu, Christopher J. Lyon and Tony Y. Hu

Lab Chip, 2024, 24, 4717
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Development of a vitrified CRISPR/Cas12b-based assay for rapid genotyping of SLCO1B1 SNPs without DNA amplification

Chen Lu, Jiayue Chen, Shengsheng Zhang, Yaru Tian, Xinyu Ying, Dan Zhang, Zhidan Luo, Xinxin Si and Ming Li

Anal. Methods, 2025, 17, 2083
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CRISPR/Cas detection with nanodevices: moving deeper into liquid biopsy

Huimin Kong, Ke Yi, Rachel L. Mintz, Bin Wang, Yanteng Xu, Yeh-Hsing Lao, Yu Tao and Mingqiang Li

Chem. Commun., 2024, 60, 2301
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Spatiotemporally regulated mitochondrial genome editing via enzyme and NIR-activated CRISPR/Cas9 nanoplatform

Fei Yang, Qianqin Ran, Jiahui Chen, Guochen Bao, Yuezhong Xian and Cuiling Zhang

Chem. Sci., 2026, 17, 5474
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Amplification-free detection of Mycobacterium tuberculosis using CRISPR-Cas12a and graphene field-effect transistors

Weiqi Wang, Huanyu Du, Changhao Dai, Hongwenjie Ma, Shi Luo, Xuejun Wang, Mingquan Guo, Derong Kong and Dacheng Wei

Nanoscale, 2025, 17, 4603
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Next-generation CRISPR/Cas-based ultrasensitive diagnostic tools: current progress and prospects

Deepak Kumar Sahel, Gangadari Giriprasad, Reena Jatyan, Sonia Guha, Aishwarya Korde, Anupama Mittal, Sunil Bhand and Deepak Chitkara

RSC Adv., 2024, 14, 32411
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Microfluidic platforms for CRISPR-based biosensing advancing molecular diagnostics from benchtop to point-of-care

Yanping Wang, Huimin Jiang, Yanyin Zhang, Qingran Yang, Yujun Song and Yanfeng Gao

Sens. Diagn., 2026, 5, 116
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One-pot isothermal amplification permits recycled activation of CRISPR/Cas12a for sensing terminal deoxynucleotidyl transferase activity

Jianguo Xu, Haidong Yang, Zhuqi Sui, Xinyue Yuan, Lee Jia and Longhua Guo

Chem. Commun., 2024, 60, 4683
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DNA-mediated precise regulation of SERS hotspots for biosensing and bioimaging

Jingjing Zhang, Chunyuan Song, Xiyu He, Jian Liu, Jie Chao and Lianhui Wang

Chem. Soc. Rev., 2025, 54, 5836
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A one-pot CRISPR-RCA strategy for ultrasensitive and specific detection of circRNA

Xinxin Ke, Ajuan Liang, Chuanxia Chen and Tao Hu

Anal. Methods, 2024, 16, 3256
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Recent developments in isothermal amplification technology for rapid detection of SARS-CoV-2

Linlin Wang, Ruitong Tang, Wentao Wang, Lingguang Bu, Jingle Sun, Yurong Fu, Meng Li and Zhengjun Yi

Anal. Methods, 2025, 17, 652
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Engineering guidelines for CRISPR diagnostics

Alexandre S. Avaro and Juan G. Santiago

Chem. Commun., 2025, 61, 18756
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Single nanoparticle analysis-based CRISPR/Cas12 bioassay for amplification-free HIV detection

Chengchao Zhang, Yanlin Chen, Xiao Chen, Xu Lin, Zili Huang, Lichun Zhang, Rui Liu and Yi Lv

Chem. Commun., 2025, 61, 8759
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TopCas: Topology‐Gated Cas12a via DNA‐RNA Chimeric Circular crRNA for Amplification‐Free Nucleic Acid Detection and Conditional Gene Editing

Shun Zhang, Wen Sun, Ting Xiao, Yali Wang, Xianlan Wu, Huiyou Chen, Ming Chen and Jun Zhang

Advanced Science, 2026
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Engineered CRISPR/Cas Ribonucleoproteins for Enhanced Biosensing and Bioimaging

Linxin Cao, Zeyuan Wang, Chunyang Lei and Zhou Nie

Anal. Chem., 2025, 97, 5866
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Evolution of CRISPR-enabled biosensors for amplification-free nucleic acid detection

Sepehr Talebian, Fariba Dehghani, Paul S. Weiss and João Conde

Trends in Biotechnology, 2024, 42, 10
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A CRISPR/Cas13a-powered catalytic hairpin assembly evanescent wave fluorescence biosensor for target amplification-free SARS-CoV-2 detection

Dan Song, Wenjuan Xu, Yuxin Zhuo, Jiayuan Liu, Anna Zhu and Feng Long

Sensors and Actuators B: Chemical, 2024, 405, 135296
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CRISPR/Cas system-based strategies for pathogenic bacteria detection

Yijia Zheng, Xinyi Sun, Xijiao Li, Jing Lei and Erqun Song

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Autocatalytic Circular DNA Powered Plasmonic CRISPR/Cas12a Platform for Ultrasensitive Non‐Nucleic Acid Target Sensing

Wang Yao, Xiaohan Xu, Xingguo Zhai, Tong Ji, Ruyi Zhang, Shenghao Xu and Xiliang Luo

Angewandte Chemie, 2026, 138
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Advances in the applications of CRISPR/Cas system for tumor molecular diagnostics

Hai-ping Wu and Zheng-lin Yang

LabMed Discovery, 2024, 1, 100011
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Multiplexed and streamlined DNA methylation detection of colorectal cancer-related genes using graphically encoded hydrogel microparticles and rolling circle amplification

Wookyoung Jang, So Jung Oh, Eun Sun Kim and Ki Wan Bong

Biosensors and Bioelectronics, 2025, 287, 117725
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A dual fluorescence channel RAA-based CRISPR-Cas12a/Cas13a system for highly sensitive detection of Gyrovirus galga1 and Gyrovirus homsa1

Dan Yu, Zhixun Xie, Yanfang Zhang, Zhiqin Xie, Qing Fan, Sisi Luo, Liji Xie, Meng Li, Tingting Zeng, Minxiu Zhang, Xiaofeng Li, You Wei, Aiqiong Wu and Lijun Wan

Virulence, 2025, 16
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One-Pot Photocontrolled CRISPR-Cas12b Coupled with Loop-Mediated Isothermal Amplification Assay for Point-of-Care Test of Mycoplasma pneumonia

Wenhui Zhu, Tao Qi, Ze Wu, Wenjie Zhong, Lizhi Yan, Jiayu Feng, Fei Jin, Wenwen Chen, Zhen Cai, Yongyu Rui, Ernestina Maria Da Costa, Quan Liu, Qiangqiang Fu and Lei Zheng

ACS Sens., 2025, 10, 9323
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Recent advances of food safety detection by nucleic acid isothermal amplification integrated with CRISPR/Cas

Jiali Qiao, Zhiying Zhao, Yaru Li, Minghui Lu, Shuli Man, Shengying Ye, Qiang Zhang and Long Ma

Critical Reviews in Food Science and Nutrition, 2024, 64, 12061
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A minimal transcription template-based amplification-free CRISPR-Cas13a strategy for DNA detection

Tianchi Zhuang, Chang Gao, Wenwu Zhao, Hairong Yu, Yun Liu, Ning Zhang, Ning Li and Minghui Ji

Biosensors and Bioelectronics, 2025, 270, 116918
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CRISPR/Cas12a: A Comprehensive Review from Structural Foundations to Applications in Nucleic Acid Precision Detection

Lu Yang and Wenzhe Ma

J. Agric. Food Chem., 2026, 74, 10689
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Dual CRISPR/Cas13a Cascade Strand Displacement-Triggered Transcription for Point-of-Care Detection of Plasmodium in Asymptomatic Malaria

Hongru Pian, Hui Wang, Honghong Wang and Zhengping Li

Anal. Chem., 2024, 96, 7524
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MnO2@Mn3O4 Heterojunction as a Coreactant Catalyst Collaborated with T-Shaped DNA Cycling–CRISPR/Cas12a Cascade Amplification for Locus-Specific N6-Methyladenosine RNA Modification Detection

Fuye Su, Heng Zhang, Chunjia Ren, Yang Jiang, Yanxia Qiao, Shengtao Zhang, Jin Liu, Sijia Li and Yan Li

Anal. Chem., 2025, 97, 24165
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Ultrasensitive Point-of-Care Detection of Protein Markers Using an Aptamer-CRISPR/Cas12a-Regulated Liquid Crystal Sensor (ALICS)

Lubin Qi, Jie Liu, Songlin Liu, Yang Liu, Yating Xiao, Zhen Zhang, Wei Zhou, Yifei Jiang and Xiaohong Fang

Anal. Chem., 2024, 96, 866
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One-pot dual-CRISPR/Cas12b-mediated dUTP-LAMP for rapid and sensitive detection of foodborne pathogens without carryover contamination

Chao Zhang, Zisheng Luo and Xingyu Lin

Food Bioscience, 2024, 59, 104069
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CRISPR-based diagnostics of different biomolecules from nucleic acids, proteins, and small molecules to exosomes

Yuanshou Zhu, Meng Zhang, Shujuan Guo, Hong Xu, Zhijun Jie and Sheng-ce Tao

ABBS, 2023, 55, 1539
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MiPRIME: an integrated and intelligent platform for mining primer and probe sequences of microbial species

Zhiming Zhang, Jing Ren, Lili Ren, Lanying Zhang, Qubo Ai, Haixin Long, Yi Ren, Kun Yang, Huiying Feng, Sabrina Li, Xu Li and Jonathan Wren

Bioinformatics, 2024, 40
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CRISPR/Cas12a-mediated DNA-AgNC label-free logical gate for multiple microRNAs’ assay

Xiaomei Mu, Jinshen Li, Shixiu Xiao, Yong Huang, Shulin Zhao and Jianniao Tian

Microchim Acta, 2024, 191
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An electrochemiluminescent imaging strategy based on CRISPR/Cas12a for ultrasensitive detection of nucleic acid

Sijian Luo, Jie Wu, Min Zhong, Jun Sun, Hang Ao, Xu Cao, Jinbo Liu and Huangxian Ju

Analytica Chimica Acta, 2024, 1324, 343040
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Design principles for LAMP–CRISPR molecular diagnostics

Carolina S. Silva, Gabriella R. Nascimento, Paulo E.O. Cruz, Rodrigo H. Arancibia, Emilia M.M. Andrade Belitardo, Thiago L.P. Castro, Livia M. Villar and Luis G.C. Pacheco

Methods, 2026, 251, 1
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Digital microfluidics methods for nucleic acid detection: A mini review

Youqiang Xing, Yan Wang, Xiang Li and Shangran Pang

Biomicrofluidics, 2024, 18
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Recent advances of nanoparticles-assisted CRISPR/Cas biosensors

Sitong Liu, Li Xu, Zhaohe Huang, Yujie Zhang, Xiaojing Pei, Shujing Li and Yifan He

Microchemical Journal, 2024, 199, 109930
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Quantitative Analysis of Cell-Free RNA at Attomolar Level Using CRISPR/Cas Digital Imaging Platform

Yanan Li, Fenglei Quan, Yige Wu, Yongyuan Zhang, Ru Xu, Yonghua Wu, Yan Liang, Junli Zhang, Hua Gao and Kaixiang Zhang

Anal. Chem., 2024, 96, 17362
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Phosphorothioate-Modified Hairpin G-Triplex Reporter-Assisted Split CRISPR/Cas12a-Powered Biosensor for “Turn-On” Fluorescent Detection of Nucleic Acid and Non-Nucleic Acid Targets

Kai Shi, Wenjie Luo, Ying Cheng, Honglei Li, Liai Peng, Xiangrui Luo, Yu Hu, Jiaheng Zhang and Jiaxuan Chen

Anal. Chem., 2025, 97, 9361
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A simple and cost-effective strategy for electrochemiluminescence spectral determination

Yifei Zhang, Dexin Gao, Hongye Yang, Wenyue Gao and Chi Wu

Analytica Chimica Acta, 2024, 1324, 343097
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Field-Deployable Detection of Genetically Modified Organisms with an Integrated Method of Loop-Mediated Isothermal Amplification and CRISPR/FnCas12a

Lu Chen, Chen Wang, Zaobing Zhu and Litao Yang

J. Agric. Food Chem., 2025, 73, 5625
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Exploring CRISPR-Cas systems for precision food authentication: Molecular tools shaping the future of traceability and integrity

M. A. Motalib Hossain, Nurhidayatul Asma Mohamad, Mohd Nasir Mohd Desa, Shuhaimi Mustafa and Amalia Mohd Hashim

Microchemical Journal, 2026, 220, 116484
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Amplification-free detection of mycoplasma pneumoniae via CRISPR-Cas12a and deep learning-optimized crRNAs on a lateral flow platform

Jian Zhou, Xue Mei Ren, Juan Gao, Rui Ping Wu, Le Chen and Zhuo Li

Journal of Pharmaceutical and Biomedical Analysis, 2026, 268, 117196
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Mpox disease, diagnosis, and point of care platforms

Nazente Atceken, Ikra Bayaki, Berk Can, Defne Yigci and Savas Tasoglu

Bioengineering & Transla Med, 2025, 10
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Temperature-boosted PAM-less activation of CRISPR-Cas12a combined with selective inhibitors enhances detection of SNVs with VAFs below 0.01%

Kena Chen, Ling Dai, Jie Zhao, Mengjun Deng, Lin Song, Dan Bai, You Wu, Xi Zhou, Yujun Yang, Shuangshuang Yang, Lin Zhao, Xueping Chen, Guoming Xie and Junjie Li

Talanta, 2023, 261, 124674
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CRISPR/Cas-based SERS detection: A win-win integration towards real-world applications

Ruibo Zhao, Yaru Li, Xinyue Li, Myunghee Kim, Xingjie Li, Shichun Pei, Shuli Man, Weipan Peng and Long Ma

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Aptamer-based dual-enzyme, amplification-free biosensor integrating CRISPR-Cas12a and Exo III for sensitive detection of ATP

Baolin Li, Yaxin Huang, Zixin Zhu, Min Zhong, Guangrong Li and Jinbo Liu

Sensing and Bio-Sensing Research, 2025, 48, 100795
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AI-powered biosensors for food safety: Resolving biomolecular challenges in complex food matrices

Kiyana Fatemi, Sie Yon Lau, Reza Fatemi, Ranil Coorey and Zoheir Heshmatipour

Food and Bioproducts Processing, 2026, 157, 125
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CRISPR technology for diagnosis and treatment of human brucellosis

Cia-Hin Lau, Xiaojun Li, Qing-Le Liang, Rui Guo, Xiangyang Ren, Zhenna Xu, Yanqiu Zhu, Ying Lai, Guoqing Liu, Yumei Huang, Weidong Wu, Haibao Zhu, Jinhui Chen and Xianpeng Zhang

Journal of Microbiological Methods, 2026, 240, 107339
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CRISPR technology - innovative diagnostics for multidrug-resistant tuberculosis

Ecaterina Cernov, Elena Chesov and Stela Racovita

Bulletin of the Academy of Sciences of Moldova. Medical Sciences, 2025, 236
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CRISPR-associated “genetic scissors” for multiplexing analysis

Chengchao Zhang, Zili Huang, Ziyan Li, Jianyu Hu, Rui Liu and Yi Lv

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Novel primer-less amplification method for detection of RNA molecules

Alicia del Prado, María I. Martínez-Jiménez, Luis Blanco and Miguel de Vega

Methods, 2025, 244, 227
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Exponential rolling circle amplification coupled with palindrome-directed bidirectional strand displacement amplification activation of dimeric CRISPR/Cas12a in miR-155 signaling

Jianguo Xu, Zhuqi Sui, Jia Zhao, Baoqiang Chen, Xiao-ming Meng and Min Yang

Sensors and Actuators B: Chemical, 2026, 448, 139020
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CRISPR-enabled sensors for rapid monitoring of environmental contaminants

Yiting Wang, Yuwei Pan, Wenchao Han, Carla Spatola Rossi, Qingxin Hui, Ying Guo, Mojisola Christiana Owoseni, Ewan McAdam, Yang-Chun Yong, Baojun Wang and Zhugen Yang

TrAC Trends in Analytical Chemistry, 2025, 184, 118128
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Entropy driven-based catalytic biosensors for bioanalysis: From construction to application-A review

Sha Yang, Xinyu Zhan, Lijia Yuan, Marc Lamy de la Chapelle, Weiling Fu and Xiang Yang

Analytica Chimica Acta, 2025, 1338, 343549
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Target-triggered hybrid chain amplified fluorescence aptasensor based on label-free dye and MnO2 nanosheets system for Escherichia coli detection

Hao Liang, Danliang Li, Shiyu Jia, Xuebing Zhang, Qinqin Bai, Ziyi Wang, Yujiao Cai, Jian Liu and Lili Chen

Microchim Acta, 2025, 192
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Pseudocapacitance-Driven Ultrasensitive Biosensing via Bimetallic MOF Synergy and DNA Amplification

Chenchen Jin, Yu Ya, Qingnian Wu, Kaili Wang, Tao Wen, Defen Feng, Ke-Jing Huang, Xuecai Tan and Jing Xu

Anal. Chem., 2025, 97, 21707
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Amplification-free, OR-gated CRISPR-Cascade reaction for pathogen detection in blood samples

Jongwon Lim, An Bao Van, Katherine Koprowski, Matthew Wester, Enrique Valera and Rashid Bashir

Proc. Natl. Acad. Sci. U.S.A., 2025, 122
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A RT-ERA-CRISPR/Cas12a assay for rapid point-of-care duck hepatitis A virus detection

Di Sun, Yukun Zhu, Mingshu Wang, Jingming Wang, Wenze Cheng, Zheng Li, Yuan Deng, Xumin Ou, Renyong Jia, Shun Chen, Dekang Zhu, Mafeng Liu, Xinxin Zhao, Qiao Yang, Ying Wu, Shaqiu Zhang, Juan Huang, Yu He, Zhen Wu and Anchun Cheng

Poultry Science, 2025, 104, 105316
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Engineering positive feedback in nucleic acid circuit Biosensors: Design and applications

Shuang Zhao, Xuesong Wang, Meilin Gong, Zuowei Xie, Jingsen Cao, Ming Chen and Kai Chang

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Amplification-free cancer diagnosis based on inhibition of Cas12a activity by site-specific 5mC-modified cfDNA

Feng Yang, Can Xu, Chunhong Li, Xinni Xiang, Yi Zhao, Cong Hu, Huan Rong, Yu He, Jingyu Li, Yu Wang, Chao Tang, Xiaoyu Liu, Renyan Li, Fei Deng and Tingxiu Xiang

Nucleic Acids Research, 2025, 53
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Gene point mutation information translation and detection: Leveraging single base extension and CRISPR/Cas12a

Zhujun Liu, Jie Xu, Shan Huang, Wei Dai, Wei Zhang, Longjie Li, Xianjin Xiao and Tongbo Wu

Biosensors and Bioelectronics, 2024, 247, 115936
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CRISPR Digital Sensing: From Micronano-Collaborative Chip to Biomolecular Detection

Xinyi Wang, Longjie Zhong, Wenbin Zhang, Pengde Wu, Minghao Wang, Dujuan Li, Linxi Dong and Gaofeng Wang

ACS Nano, 2025, 19, 20427
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A novel CRISPR-driven Fe SANs-sensitized hydrogel biosensor for ultrasensitive detection of microcystin synthetase gene E (mcyE) and early warning of microcystin-LR

Pian Wu, Heng Dai, Jun Liu, Yuan Tian, Xiaolong Qu, Zongwu Meng, Tianhan Kai and Ping Ding

Chemical Engineering Journal, 2025, 521, 166408
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A Relay CRISPR/Cas12a System for Extraction-Free and Ultrasensitive Detection of Staphylococcus aureus in Complex Food Samples

Kun Wang, Wanli Wang, Yufeng Hu, Xiaoyang Li, Haoyu Yu, Xin Xia, Tianhan Kai and Mei Wen

Anal. Chem., 2026, 98, 1657
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From Lab to Market: Challenges in Translating Biosensor Innovations to Benefit Agricultural Animal Health

Zhen Qian, Tom Kasputis, Qiaoqiao Ci, Yuanzhi Bian and Chenming Zhang

Biosensors and Bioelectronics, 2026, 118683
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RPA-CRISPR/Cas12a-coupled microfluidic biosensor enabling on-site, sensitive quantification of Vibrio parahaemolyticus

Jiehong Fang, Qi Chen, Meijuan Ran, Ran Chen, Wenyu Chen, Jing Cui, Jing Wang, Kai Zhong, Liwen Shi, Chenze Lu and Han Jiang

Biosensors and Bioelectronics, 2026, 296, 118327
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Multiplexed CRISPR/Cas and Argonaute detection technology: strategies, challenges, and perspectives

Yaru Li, Lu Zhao, Wen Kang, Long Ma, Yunfeng Bai and Feng Feng

TrAC Trends in Analytical Chemistry, 2025, 191, 118329
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The progress of CRISPR/Cas-based RNA detection

Mohammed AL-Dulaiei, Ying Li, Jinyan Long, Yang Yu, Tao Ma and Xiaolan Yang

Microchemical Journal, 2025, 218, 115427
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A systematic review of rapid SARS-CoV-2 detection methods for mitigating future outbreaks of emerging or historical pathogens

Yuer Wang, Jiteng Xiao, Junqing Wei, Yadan Peng, Mengzhe Li and Yigang Tong

Microchemical Journal, 2024, 204, 111126
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Label-free detection of let-7a using AuNPs-coated polystyrene microparticles

Bonghyeok Eom, Sunghyun Ki, Soongwon Cho and Dong-Ku Kang

Microchemical Journal, 2025, 216, 114590
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Nanoplasmonic real-time RT-RPA and CRISPR/Cas12a detection for rapid point-of-care molecular diagnostics

Eun-Sil Yu, Hyowon Jang, Jae-Myeong Kwon, Hyejeong Jeong, Jaehyeok Park, Byeong-Hoon Kang, Donggee Rho, Sungmin Son, Taejoon Kang and Ki-Hun Jeong

Biosensors and Bioelectronics, 2026, 294, 118216
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Establishment of One-Pot ERA-CRISPR/Cas12a-Based Rapid Visual Assays and a TaqMan Quantitative PCR Assay for Lactococcus garvieae

Haoyu Wang, Heng Sun, Feiming Chen, Zhiyuan Huang, Yu Chen, Xiaofeng Chen, Dogbey Rejoice Abla, Zhi Zhang, Huajian Lin, Liqun Wang and Yucong Huang

Microorganisms, 2026, 14, 830
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Recent progress of biosensing techniques for environmental antibiotic resistance genes

Fangqi Wu, Qi Zhu, Liang Zhang, Wei Shen, Jingming Gong, Hian Kee Lee and Sheng Tang

TrAC Trends in Analytical Chemistry, 2025, 191, 118380
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Super signal-enhancement biosensing platform for precise target recognition based on rolling circle-hybridization chain dual linear cascade amplification technology

Danyao Tang, Chunyuan Xiong, YeYu Wu, Hu Luo, Jun Yan, Ke-Jing Huang, Xuecai Tan and Yu Ya

Talanta, 2025, 285, 127321
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Multicolor Amplification-Free RNA Detection with Cas13a and Cas13b

Hajime Shinoda, Asami Makino, Mami Yoshimura, Noriko Minagawa, Tatsuya Iida, Masahiro Nakano, Takeshi Noda, Masashi Toyoda and Rikiya Watanabe

Anal. Chem., 2026, 98, 4705
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Harnessing multiplex crRNA enables an amplification-free/CRISPR-Cas12a-based diagnostic methodology for Nosema bombycis

Huarui Zhang, Huijuan Zhao, Lu Cao, Bin Yu, Junhong Wei, Guoqing Pan, Jialing Bao, Zeyang Zhou and Chengshu Wang

Microbiol Spectr, 2024, 12
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Advanced Cancer Liquid Biopsy Platform for miRNA Detection in Extracellular Vesicles Using CRISPR/Cas13a and Gold Nanoarrays

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Programmable AIESTA: All-in-One Isothermal Enzymatic Signal Transduction Amplifier for Portable Profiling

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B Doped Zn Single‐Atom Nanozyme With Enhanced Oxidase‐Like Activity Combined CRISPR/Cas13a System for RNA Sensing

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Nanoparticle-based biosensor integrated with CRISPR/Cas12b platform for sensitive and visual identification of hepatitis B virus pregenomic RNA in chronic hepatitis B patients

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RNase H-Driven crRNA Switch Circuits for Rapid and Sensitive Detection of Various Analytical Targets

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Automatic Microfluidic Harmonized RAA-CRISPR Diagnostic System for Rapid and Accurate Identification of Bacterial Respiratory Tract Infections

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Detection of tomato brown rugose fruit virus through CRISPR-Cas12a and CRISPR-Cas9 systems

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PathoGD: an integrative genomics approach to primer and guide RNA design for CRISPR-based diagnostics

Soo Jen Low, Matthew O’Neill, William J. Kerry, Natasha Wild, Marcelina Krysiak, Yi Nong, Francesca Azzato, Eileen Hor, Lewis Williams, George Taiaroa, Eike Steinig, Shivani Pasricha and Deborah A. Williamson

Commun Biol, 2025, 8
DOI: 10.1038/s42003-025-07591-1

The nucleic acid detection using CRISPR/Cas biosensing system with micro-nano modality for point-of-care applications

Liang Zhao, Zihao Zhao, Ning Li and Xiayan Wang

Talanta, 2025, 286, 127457
DOI: 10.1016/j.talanta.2024.127457

Entropy‐Driven Strand Displacements Around DNA Tetrahedron for Sensitive Detection and Intracellular Imaging of mRNA

Jinwen Zhu, Xiaolin Qu, Yuan Zhuang and Peng Miao

Small Structures, 2024, 5
DOI: 10.1002/sstr.202300420

Harnessing CRISPR/Cas Systems for DNA and RNA Detection: Principles, Techniques, and Challenges

Heyjin Son

Biosensors, 2024, 14, 460
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Red and blue microspheres based immunoassay combined with RPA and Cas12a for dual-detection of nucleic acid from foodborne pathogens

Lemei Zhu, Jinbin Wang, Xi Li, Hui Zhen, Ziyi Gu, Laifa Wang, Pingping Bing and Haijuan Zeng

Microchim Acta, 2026, 193
DOI: 10.1007/s00604-026-07847-5

Spherical nucleic acid enzyme programmed network to accelerate CRISPR assays for electrochemiluminescence biosensing applications

Yi Li, Mei-Ling Liu, Wen-Bin Liang, Ying Zhuo and Xiao-Jing He

Biosensors and Bioelectronics, 2023, 238, 115589
DOI: 10.1016/j.bios.2023.115589

Novel Multimode Assay Based on Asymmetrically Competitive CRISPR and Raman Barcode Spectra for Multiple Hepatocellular Carcinoma Biomarkers Detection

Hao Jiang, Cheng Qian, Yulin Deng, Xuefei Lv, Ying Liu, Anyi Li and Xiaoqiong Li

Anal. Chem., 2024, 96, 20004
DOI: 10.1021/acs.analchem.4c04593

A portable, one-tube CRISPR/Cas12a-based biosensor for sensitive and colorimetric detection of norovirus in foods without pre-amplification

Caihong Zhou, Jingjing Fan, Liangyuan Wang, Baoxue Gao, Chunhui Wei, Long Ma and Lijuan Yin

Microchemical Journal, 2025, 212, 113469
DOI: 10.1016/j.microc.2025.113469

A Chimeric Photo-Controllable CRISPR/Cas12a System for Universal and Fast Diagnostics

Xinrong Yan, Bin Liu, Shuguang Zhou, Yanjun Fan, Shijiong Wei, Dehui Qiu, Henglong Xiang, Jiahang Zhou, Jean-Louis Mergny, David Monchaud, Huangxian Ju and Jun Zhou

Anal. Chem., 2025, 97, 24634
DOI: 10.1021/acs.analchem.5c04782

Fully Integrated Microfluidic Platform for Multiplexed Detection of Hunov by a Dynamic Confined‐Space‐Implemented One‐Pot Rpa‐Lamp System

Fumin Chen, Chenang Lyu, Zhao Li, Leshan Xiu, Huimin Li, Yi Xie, Runzhen Cao, Qinqin Hu and Kun Yin

Advanced Science, 2024, 11
DOI: 10.1002/advs.202306612

Degradation of Reporter Molecules Imposes a Fundamental Limit of Detection on CRISPR Diagnostics

Alexandre S. Avaro, Andrew D. Griffiths and Juan G. Santiago

Anal. Chem., 2025, 97, 13192
DOI: 10.1021/acs.analchem.5c01060

Asymmetric CRISPR-Cas12a powered electrochemical aptasensor for clenbuterol detection based on competitive gRNA mediated cascade signal amplification

Sujun Liu and Kai Shi

Food Chemistry, 2025, 464, 141928
DOI: 10.1016/j.foodchem.2024.141928

Argonaute-DNAzyme tandem biosensing for highly sensitive and simultaneous dual-gene detection of methicillin-resistant Staphylococcus aureus

Yaru Li, Xiaoqin Tang, Nan Wang, Zhiying Zhao, Shuli Man, Lei Zhu and Long Ma

Biosensors and Bioelectronics, 2024, 244, 115758
DOI: 10.1016/j.bios.2023.115758

Fluorescence biosensor for ultrasensitive detection of the available lead based on target biorecognition-induced DNA cyclic assembly

Junhua Chen, Manjia Chen, Hui Tong, Fei Wu, Yizhang Liu and Chengshuai Liu

Science of The Total Environment, 2023, 905, 167253
DOI: 10.1016/j.scitotenv.2023.167253

CRISPR-enabled point-of-care diagnostics for SARS-CoV-2 variant surveillance: design principles for adapting molecular detection to viral evolution

Avin Mohammadnejad, Long Ma, Ewa Goldys and Fei Deng

TrAC Trends in Analytical Chemistry, 2026, 199, 118808
DOI: 10.1016/j.trac.2026.118808

Recent advances in CRISPR/Cas12a activation modes for versatile and robust diagnostics

Siying Xie, Shulin Wang, Yilin Hu, Wei Lv and Bingzhi Li

TrAC Trends in Analytical Chemistry, 2026, 200, 118829
DOI: 10.1016/j.trac.2026.118829