Issue 6, 2024

Recent progress on the CRISPR/Cas system in optical biosensors

Abstract

Clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated (Cas) protein systems are adaptive immune systems unique to archaea and bacteria, with the characteristics of targeted recognition and gene editing to resist the invasion of foreign nucleic acids. Biosensors combined with the CRISPR/Cas system and optical detection technology have attracted much attention in medical diagnoses, food safety, agricultural progress, and environmental monitoring owing to their good sensitivity, high selectivity, and fast detection efficiency. In this review, we introduce the mechanism of CRISPR/Cas systems and developments in this area, followed by summarizing recent progress on CRISPR/Cas system-based optical biosensors combined with colorimetric, fluorescence, electrochemiluminescence and surface-enhanced Raman scattering optical techniques in various fields. Finally, we discuss the challenges and future perspectives of CRISPR/Cas systems in optical biosensors.

Graphical abstract: Recent progress on the CRISPR/Cas system in optical biosensors

Article information

Article type
Critical Review
Submitted
01 dec 2023
Accepted
05 jan 2024
First published
23 jan 2024

Anal. Methods, 2024,16, 798-816

Recent progress on the CRISPR/Cas system in optical biosensors

B. Li, G. Zhai, Y. Dong, L. Wang and P. Ma, Anal. Methods, 2024, 16, 798 DOI: 10.1039/D3AY02147E

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