Issue 9, 2023

Spatiotemporal dynamics of DNA nanocage uptake in zebrafish embryos for targeted tissue bioimaging applications

Abstract

Three-dimensional DNA nanocages have attracted significant attention for various biomedical applications including targeted bioimaging in vivo. Despite the numerous advantages, the use and in vivo exploration of DNA nanocages are limited as the cellular targeting and intracellular fate of these DNA nanocages within various model systems have not been explored well. Herein, using a zebrafish model system, we provide a detailed understanding of time-, tissue- and geometry-dependent DNA nanocage uptake in developing embryos and larvae. Of all the geometries tested, tetrahedrons showed significant internalization in 72 hours post-fertilized larvae upon exposure, without disturbing the expression of genes involved in embryo development. Our study provides a detailed understanding of the time and tissue-specific uptake of DNA nanocages in the zebrafish embryos and larvae. These findings will provide valuable insights into the internalization and biocompatible potential of DNA nanocages and will help to predict their candidature for biomedical applications.

Graphical abstract: Spatiotemporal dynamics of DNA nanocage uptake in zebrafish embryos for targeted tissue bioimaging applications

Supplementary files

Article information

Article type
Paper
Submitted
10 dec 2022
Accepted
02 apr 2023
First published
17 apr 2023
This article is Open Access
Creative Commons BY-NC license

Nanoscale Adv., 2023,5, 2558-2564

Spatiotemporal dynamics of DNA nanocage uptake in zebrafish embryos for targeted tissue bioimaging applications

K. Kansara, A. Mansuri, A. Rajwar, P. Vaswani, R. Singh, A. Kumar and D. Bhatia, Nanoscale Adv., 2023, 5, 2558 DOI: 10.1039/D2NA00905F

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