Issue 29, 2021

Enzyme-activatable fluorescent probes for β-galactosidase: from design to biological applications

Abstract

β-Galactosidase (β-gal), a typical hydrolytic enzyme, is a vital biomarker for cell senescence and primary ovarian cancers. Developing precise and rapid methods to monitor β-gal activity is crucial for early cancer diagnoses and biological research. Over the past decade, activatable optical probes have become a powerful tool for real-time tracking and in vivo visualization with high sensitivity and specificity. In this review, we summarize the latest advances in the design of β-gal-activatable probes via spectral characteristics and responsiveness regulation for biological applications, and particularly focus on the molecular design strategy from turn-on mode to ratiometric mode, from aggregation-caused quenching (ACQ) probes to aggregation-induced emission (AIE)-active probes, from near-infrared-I (NIR-I) imaging to NIR-II imaging, and from one-mode to dual-mode of chemo-fluoro-luminescence sensing β-gal activity.

Graphical abstract: Enzyme-activatable fluorescent probes for β-galactosidase: from design to biological applications

Article information

Article type
Review Article
Submitted
13 apr 2021
Accepted
27 jun 2021
First published
16 jul 2021
This article is Open Access

All publication charges for this article have been paid for by the Royal Society of Chemistry
Creative Commons BY-NC license

Chem. Sci., 2021,12, 9885-9894

Enzyme-activatable fluorescent probes for β-galactosidase: from design to biological applications

Y. Yao, Y. Zhang, C. Yan, W. Zhu and Z. Guo, Chem. Sci., 2021, 12, 9885 DOI: 10.1039/D1SC02069B

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