Issue 2, 2021

Revision in the first steps of the biosynthesis of the red antibiotic prodigiosin: use of a synthetic thioester to validate a new intermediate

Abstract

A biosynthetic pathway for the red-antibiotic, prodigiosin, was proposed over a decade ago but not all the suggested intermediates could be detected experimentally. Here we show that a thioester that was not originally included in the pathway is an intermediate. In addition, the enzyme PigE was originally described as a transaminase but we present evidence that it also catalyses the reduction of the thioester intermediate to its aldehyde substrate.

Graphical abstract: Revision in the first steps of the biosynthesis of the red antibiotic prodigiosin: use of a synthetic thioester to validate a new intermediate

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Article information

Article type
Paper
Submitted
30 sep 2020
Accepted
05 jan 2021
First published
15 jan 2021
This article is Open Access
Creative Commons BY license

RSC Chem. Biol., 2021,2, 551-555

Revision in the first steps of the biosynthesis of the red antibiotic prodigiosin: use of a synthetic thioester to validate a new intermediate

M. Couturier, H. D. Bhalara, R. E. Monson, G. P. C. Salmond and F. J. Leeper, RSC Chem. Biol., 2021, 2, 551 DOI: 10.1039/D0CB00173B

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