Issue 11, 2020

Exploration of production of C14 and C15 bacillomycin D homologues with enzymatic hydrolysis from maize straws using fed-batch fermentation by Bacillus subtilis NS-174

Abstract

A strain with strong antifungal activity, Bacillus subtilis NS-174, was identified and the antifungal compounds were purified and structurally analyzed by high performance liquid chromatography-mass spectrometry/mass spectrometry (HPLC-MS/MS). The effects of the enzymatic hydrolysis and the fermentation strategies on production of the antifungal compounds were also investigated. The results showed that the antifungal compounds were characterized to be C14 and C15 bacillomycin D homologues (C14–15BDs). When 400 mL of the cellulase hydrolysates from the maize straws residues (CHMSRs) was fed into 1.0 L of the batch fermentation broth at 60 h, an output of C14–15BDs (OC14–15BDs) was obtained as 44.84 mg gmaize straws−1. Moreover, the fed-batch fermentation was beneficial for the increase of the transcriptional activities of C14–15BDs synthetase genes by up-regulating expression of comA, sigmaH and spo0A, which caused the elevation of C14–15BDs production. The present work provided an effective solution for lipopeptide production in Bacillus subtilis.

Graphical abstract: Exploration of production of C14 and C15 bacillomycin D homologues with enzymatic hydrolysis from maize straws using fed-batch fermentation by Bacillus subtilis NS-174

Article information

Article type
Paper
Submitted
15 dec 2019
Accepted
06 feb 2020
First published
12 feb 2020
This article is Open Access
Creative Commons BY-NC license

RSC Adv., 2020,10, 6725-6734

Exploration of production of C14 and C15 bacillomycin D homologues with enzymatic hydrolysis from maize straws using fed-batch fermentation by Bacillus subtilis NS-174

S. Qian, X. Li, L. Sun, Y. Shen, Q. Ren, E. Diao and Z. Lu, RSC Adv., 2020, 10, 6725 DOI: 10.1039/C9RA10536K

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