Issue 10, 2018

Multicolor monitoring of cellular organelles by single wavelength excitation to visualize the mitophagy process

Abstract

Multiplexed cellular organelle imaging using single wavelength excitation is highly desirable for unravelling cellular functions but remains challenging. This requires the design of organelle specific fluorophores with distinct emission but similar absorption. Herein, we present two unique aggregation-induced emission (AIE) probes to track mitochondria and lysosomes simultaneously with emission colors that can be distinguished from that of the nucleus stain Hoechst 33342 upon single wavelength excitation. Compared to conventional organelle stains, the two AIE probes have larger Stokes shifts and higher photostability, which endow them with the capability to monitor bioprocesses, such as mitophagy with strong and sustained fluorescent signals. Moreover, both probes can also stain intracellular organelles in zebrafish larvae with good cell-penetrating capabilities, showing their great potential to monitor bioprocesses in vivo.

Graphical abstract: Multicolor monitoring of cellular organelles by single wavelength excitation to visualize the mitophagy process

Supplementary files

Article information

Article type
Edge Article
Submitted
23 okt 2017
Accepted
01 feb 2018
First published
02 feb 2018
This article is Open Access

All publication charges for this article have been paid for by the Royal Society of Chemistry
Creative Commons BY-NC license

Chem. Sci., 2018,9, 2756-2761

Multicolor monitoring of cellular organelles by single wavelength excitation to visualize the mitophagy process

F. Hu, X. Cai, P. N. Manghnani, Kenry, W. Wu and B. Liu, Chem. Sci., 2018, 9, 2756 DOI: 10.1039/C7SC04585A

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