Issue 3, 2017

Responsive hetero-organelle partition conferred fluorogenic sensing of mitochondrial depolarization

Abstract

Malfunctioning organelles are often difficult to probe with classical organelle-homing sensors owing to disruption of physiological organelle-probe affinity. We herein report the use of a responsive hetero-organelle partition and signal activable probe (RC-TPP) for detecting mitochondrial depolarization, a pathologically relevant event featuring loss of the electrical potentials across the mitochondrial membrane (ΔΨm). Partitioned in mitochondria to give blue fluorescence, RC-TPP relocates into lysosomes upon mitochondrial depolarization and exhibits red fluorescence triggered by lysosomal acidity, enabling determination of autophagy relevant mitochondrial depolarization and the chronological sequence of mitochondrial depolarization and lysosomal neutralization in distinct cell death signalling pathways. As an alternative to classic homo-organelle specific molecular systems, this hetero-organelle responsive approach provides a new perspective from which to study dysfunctional organelles.

Graphical abstract: Responsive hetero-organelle partition conferred fluorogenic sensing of mitochondrial depolarization

Supplementary files

Article information

Article type
Edge Article
Submitted
16 sep 2016
Accepted
08 nov 2016
First published
08 nov 2016
This article is Open Access

All publication charges for this article have been paid for by the Royal Society of Chemistry
Creative Commons BY-NC license

Chem. Sci., 2017,8, 1915-1921

Responsive hetero-organelle partition conferred fluorogenic sensing of mitochondrial depolarization

Z. Xue, H. Zhao, J. Liu, J. Han and S. Han, Chem. Sci., 2017, 8, 1915 DOI: 10.1039/C6SC04158B

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