Issue 1, 2017

MnO2 nanosheet mediated “DD–A” FRET binary probes for sensitive detection of intracellular mRNA

Abstract

The donor donor–acceptor (DD–A) FRET model has proven to have a higher FRET efficiency than donor–acceptor acceptor (D–AA), donor–acceptor (D–A), and donor donor–acceptor acceptor (DD–AA) FRET models. The in-tube and in-cell experiments clearly demonstrate that the “DD–A” FRET binary probes can indeed increase the FRET efficiency and provide higher imaging contrast, which is about one order of magnitude higher than the ordinary “D–A” model. Furthermore, MnO2 nanosheets were employed to deliver these probes into living cells for intracellular TK1 mRNA detection because they can adsorb ssDNA probes, penetrate across the cell membrane and be reduced to Mn2+ ions by intracellular GSH. The results indicated that the MnO2 nanosheet mediated “DD–A” FRET binary probes are capable of sensitive and selective sensing gene expression and chemical-stimuli changes in gene expression levels in cancer cells. We believe that the MnO2 nanosheet mediated “DD–A” FRET binary probes have the potential as a simple but powerful tool for basic research and clinical diagnosis.

Graphical abstract: MnO2 nanosheet mediated “DD–A” FRET binary probes for sensitive detection of intracellular mRNA

Supplementary files

Article information

Article type
Edge Article
Submitted
18 jul 2016
Accepted
01 sep 2016
First published
05 sep 2016
This article is Open Access

All publication charges for this article have been paid for by the Royal Society of Chemistry
Creative Commons BY-NC license

Chem. Sci., 2017,8, 668-673

MnO2 nanosheet mediated “DD–A” FRET binary probes for sensitive detection of intracellular mRNA

M. Ou, J. Huang, X. Yang, K. Quan, Y. Yang, N. Xie and K. Wang, Chem. Sci., 2017, 8, 668 DOI: 10.1039/C6SC03162E

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