Issue 6, 2011
From the journal:

Journal of Analytical Atomic Spectrometry

A dual-labelling strategy for integrated ICPMS and LIF for the determination of peptides†

Zhubao Zhang,a   Xiaowen Yan,a   Ming Xu,a   Limin Yanga  and  Qiuquan Wang*ab  

* Corresponding authors

a Department of Chemistry & the Key Laboratory of Analytical Sciences, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, China
E-mail: qqwang@xmu.edu.cn
Fax: +86 592 2187400
Tel: +86 592 2181796

b State Key Laboratory of Marine Environmental Science, Xiamen University, Xiamen, China

Abstract

In this proof-of-concept study, a novel dual-labelling strategy for conjugating –SH and –NH2 of a peptide was developed, in which an element tag (1,4,7,10-tetraazacyclododecane-1,4,7-trisacetic acid-10-maleimidoethylacetamide loaded with europium) and a fluorescent tag (fluorescein isothiocycanate) were employed. Its feasibility was demonstrated using HPLC-UV/ESI-MS for evaluating labelling-efficiency and HPLC-ICPMS with 153Eu isotope dilution as well as CE-LIF for the determination of peptides.

Graphical abstract: A dual-labelling strategy for integrated ICPMS and LIF for the determination of peptides

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Article information

DOI
https://doi.org/10.1039/C1JA00010A
Article type
Communication
Submitted
06 jan 2011
Accepted
18 mrt 2011
First published
01 apr 2011

J. Anal. At. Spectrom., 2011,26, 1175-1177

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A dual-labelling strategy for integrated ICPMS and LIF for the determination of peptides

Z. Zhang, X. Yan, M. Xu, L. Yang and Q. Wang, J. Anal. At. Spectrom., 2011, 26, 1175 DOI: 10.1039/C1JA00010A

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