Issue 20, 2023

Acoustic quasi-periodic bioassembly based diverse stem cell arrangements for differentiation guidance

Abstract

Arrangement patterns and geometric cues have been demonstrated to influence cell function and fate, which calls for efficient and versatile cell patterning techniques. Despite constant achievements that mainly focus on individual cells and uniform cell patterns, simultaneously constructing cellular arrangements with diverse patterns and positional relationships in a flexible and contact-free manner remains a challenge. Here, stem cell arrangements possessing multiple geometries and structures are proposed based on powerful and diverse pattern-building capabilities of quasi-periodic acoustic fields, with advantages of rich patterns and structures and flexibility in structure modulation. Eight-fold waves' interference produces regular potentials that result in higher rotational symmetry and more complex arrangement of geometric units. Moreover, through flexible modulation of the phase relations among these wave vectors, a wide variety of cellular pattern units are arranged in this potential, such as circular-, triangular- and square-shape, simultaneously. It is proved that these diverse cellular patterns conveniently build human mesenchymal stem cell (hMSC) models, for research on the effect of cellular arrangement on stem cell differentiation. This work fills the gap of acoustic cell patterning in quasi-periodic patterns and shows promising potential in tissue engineering and regenerative medicine.

Graphical abstract: Acoustic quasi-periodic bioassembly based diverse stem cell arrangements for differentiation guidance

Supplementary files

Article information

Article type
Paper
Submitted
23 mei 2023
Accepted
21 sep 2023
First published
29 sep 2023

Lab Chip, 2023,23, 4413-4421

Acoustic quasi-periodic bioassembly based diverse stem cell arrangements for differentiation guidance

X. Gao, X. Hu, D. Yang, Q. Hu, J. Zheng, S. Zhao, C. Zhu, X. Xiao and Y. Yang, Lab Chip, 2023, 23, 4413 DOI: 10.1039/D3LC00448A

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