Issue 9, 2023

Facilitation of axonal transcriptome analysis with quantitative microfluidic devices

Abstract

Microfluidic chambers are powerful tools for studying axonal mRNA localization and translation in neurons. In addition to specific manipulation and measurements of axons, microfluidic chambers are used for collecting axonal materials to perform axonal transcriptome analysis. However, traditional bipartite and tripartite chambers have limitations either in purity or quantity of collected axons. Here, we improved the design of traditional chambers. Moreover, we developed two new quantitative chambers, multi-compartmental quantitative bipartite chamber (MQBC) and long quantitative tripartite chamber (LQTC). Compared with the traditional chambers, MQBC and LQTC could dramatically increase the efficiency in collecting axonal RNA. Finally, we applied these chambers to do comparative axon transcriptome analysis of different types of neurons. Thus, our newly designed quantitative chambers significantly improve axon collection efficiency and facilitate axonal transcriptome analysis.

Graphical abstract: Facilitation of axonal transcriptome analysis with quantitative microfluidic devices

Supplementary files

Article information

Article type
Paper
Submitted
27 dec 2022
Accepted
04 apr 2023
First published
05 apr 2023

Lab Chip, 2023,23, 2217-2227

Facilitation of axonal transcriptome analysis with quantitative microfluidic devices

Z. Yang, J. Yu, J. Zhang, H. Song, H. Ye, J. Liu, N. Wang, P. Che, G. Long, Y. Wang, J. Park and S. Ji, Lab Chip, 2023, 23, 2217 DOI: 10.1039/D2LC01183B

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements