Issue 8, 2012

A facile in situ microfluidic method for creating multivalent surfaces: toward functional glycomics

Abstract

An in situ method of modifying the chemistry and topology of microfluidic surfaces in order to mimic the cellular environment is described. The binding of functionalised microbeads to microfluidic channels allows the surface-to-volume ratio of the system, and thus the number of biomolecules available for reaction, to be vastly increased, thereby enhancing the sensitivity of biochemical analyses. The sensitivity and specificity of the technique were first investigated via the study of carbohydrate–protein interactions. Beads featuring hydrazide moieties were adhered to the channel surface, after which carbohydrates (galactose and mannose) were bound to the beads in situ and reacted with fluorescently labelled proteins. Results showed a six-fold increase in fluorescent signal compared to the same process performed on a glass surface without the presence of beads, thereby demonstrating the increase in valence afforded by the method. In a subsequent study, beads, modified with galactose moieties via the in situ functionalisation technique, were used to perform studies of colon tumour cells from a cell sample. Here, the carcinoma cells exhibited superior adhesion than the normal cells due to an increased expression of active galactose receptors, thereby demonstrating the success of the biofunctionalisation method for investigating cellular mechanisms.

Graphical abstract: A facile in situ microfluidic method for creating multivalent surfaces: toward functional glycomics

Article information

Article type
Paper
Submitted
08 dec 2011
Accepted
26 jan 2012
First published
31 jan 2012

Lab Chip, 2012,12, 1500-1507

A facile in situ microfluidic method for creating multivalent surfaces: toward functional glycomics

G. Simone, P. Neuzil, G. Perozziello, M. Francardi, N. Malara, E. Di Fabrizio and A. Manz, Lab Chip, 2012, 12, 1500 DOI: 10.1039/C2LC21217J

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements