Issue 10, 2023

Anti-PEG antibodies enriched in the protein corona of PEGylated nanocarriers impact the cell uptake

Abstract

Poly(ethylene glycol) (PEG) is the gold standard used to reduce unspecific protein adsorption and prolong nanocarrier circulation time. However, this stealth effect could be counteracted by the increasing prevalence of anti-PEG antibodies in the bloodstream. Up to now, the presence of anti-PEG antibodies in the protein corona and their effect on cell uptake has not been investigated yet. Our results showed a high concentration and prevalence of anti-PEG antibodies in the German population. PEGylated nanocarriers exhibited a higher level of anti-PEG antibodies in the protein corona compared to non-PEGylated, which lead to higher uptake in macrophages. Consequently, the anti-PEG antibodies in the protein corona could mitigate the stealth effect of PEG, leading to accelerated blood clearance and unwanted side effects.

Graphical abstract: Anti-PEG antibodies enriched in the protein corona of PEGylated nanocarriers impact the cell uptake

Supplementary files

Article information

Article type
Communication
Submitted
26 mei 2023
Accepted
01 aug 2023
First published
04 aug 2023
This article is Open Access
Creative Commons BY license

Nanoscale Horiz., 2023,8, 1377-1385

Anti-PEG antibodies enriched in the protein corona of PEGylated nanocarriers impact the cell uptake

M. F. S. Deuker, V. Mailänder, S. Morsbach and K. Landfester, Nanoscale Horiz., 2023, 8, 1377 DOI: 10.1039/D3NH00198A

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