Issue 4, 2023

Magnetically localized and wash-free fluorescence immunoassay (MLFIA): proof of concept and clinical applications

Abstract

Immunoassays are used for many applications in various markets, from clinical diagnostics to the food industry, generally relying on gold-standard ELISAs that are sensitive, robust, and cheap but also time-consuming and labour intensive. As an alternative, we propose here the magnetically localized and wash-free fluorescence immunoassay (MLFIA): a no-wash assay to directly measure a biomolecule concentration, without mixing nor washing steps. To do so, a fluorescence no-wash measurement is performed to generate a detectable signal. It consists of a differential measurement between the fluorescence of fluorophores bound to magnetic nanoparticles specifically captured by micro-magnets against the residual background fluorescence of unbound fluorophores. Targeted biomolecules (antibodies or antigens) are locally concentrated on micro-magnet lines, with the number of captured biomolecules quantitatively measured without any washing step. The performance of the MLFIA platform is assessed and its use is demonstrated with several biological models as well as clinical blood samples for HIV, HCV and HBV detection, with benchmarking to standard analyzers of healthcare laboratories. Thus, we demonstrated for the first time the versatility of the innovative MLFIA platform. We highlighted promising performances with the successful quantitative detection of various targets (antigens and antibodies), in different biological samples (serum and plasma), for different clinical tests (HCV, HBV, HIV).

Graphical abstract: Magnetically localized and wash-free fluorescence immunoassay (MLFIA): proof of concept and clinical applications

Supplementary files

Article information

Article type
Paper
Submitted
03 okt 2022
Accepted
05 dec 2022
First published
26 jan 2023
This article is Open Access
Creative Commons BY-NC license

Lab Chip, 2023,23, 645-658

Magnetically localized and wash-free fluorescence immunoassay (MLFIA): proof of concept and clinical applications

S. Delshadi, M. Fratzl, O. Ramel, P. Bigotte, P. Kauffmann, D. Kirk, V. Masse, M. P. Brenier-Pinchart, H. Fricker-Hidalgo, H. Pelloux, F. Bruckert, C. Charrat, O. Cugat, N. M. Dempsey, T. Devillers, P. Halfon, A. Leroy, M. Weidenhaupt and P. N. Marche, Lab Chip, 2023, 23, 645 DOI: 10.1039/D2LC00926A

This article is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported Licence. You can use material from this article in other publications, without requesting further permission from the RSC, provided that the correct acknowledgement is given and it is not used for commercial purposes.

To request permission to reproduce material from this article in a commercial publication, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party commercial publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements