Issue 1, 2022

A sample-to-answer electrochemical biosensor system for biomarker detection

Abstract

Biomarker detection is critical for the diagnosis and treatment of numerous diseases. Typically, target biomarkers in blood samples are measured through tests conducted at centralized laboratories. Testing at central laboratories increases wait times for results, in turn increasing healthcare costs and negatively impacting patient outcomes. Alternatively, point-of-care platforms enable the rapid measurement of biomarkers, expand testing location capabilities and mitigate manual processing steps through integration and automation. However, many of these systems focus on sample detection rather than the equally important sample preparation. Here we present a fully integrated and automated sample-to-answer electrochemical biosensing platform which incorporates each aspect of the biomarker testing workflow from blood collection to sample preparation to assay operation and readout. The system combines a commercial microneedle blood sampling device with membrane-based plasma filtration upstream of a bead-based electrochemical immunoassay. We characterize the high separation efficiency (>99%) and low non-specific binding of the whole blood-to-plasma filtration membrane under a range of operating conditions. We demonstrate a full sample-to-answer workflow through the analysis of interlukin-6-spiked blood samples.

Graphical abstract: A sample-to-answer electrochemical biosensor system for biomarker detection

Supplementary files

Article information

Article type
Paper
Submitted
08 okt 2021
Accepted
30 nov 2021
First published
10 dec 2021
This article is Open Access
Creative Commons BY license

Lab Chip, 2022,22, 100-107

A sample-to-answer electrochemical biosensor system for biomarker detection

K. Kikkeri, D. Wu and J. Voldman, Lab Chip, 2022, 22, 100 DOI: 10.1039/D1LC00910A

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