Issue 6, 2022

A library of Rhodamine6G-based pH-sensitive fluorescent probes with versatile in vivo and in vitro applications

Abstract

Acidic pH is critical to the function of the gastrointestinal system, bone-resorbing osteoclasts, and the endolysosomal compartment of nearly every cell in the body. Non-invasive, real-time fluorescence imaging of acidic microenvironments represents a powerful tool for understanding normal cellular biology, defining mechanisms of disease, and monitoring for therapeutic response. While commercially available pH-sensitive fluorescent probes exist, several limitations hinder their widespread use and potential for biologic application. To address this need, we developed a novel library of pH-sensitive probes based on the highly photostable and water-soluble fluorescent molecule, Rhodamine 6G. We demonstrate versatility in terms of both pH sensitivity (i.e., pKa) and chemical functionality, allowing conjugation to small molecules, proteins, nanoparticles, and regenerative biomaterial scaffold matrices. Furthermore, we show preserved pH-sensitive fluorescence following a variety of forms of covalent functionalization and demonstrate three potential applications, both in vitro and in vivo, for intracellular and extracellular pH sensing. Finally, we develop a computation approach for predicting the pH sensitivity of R6G derivatives, which could be used to expand our library and generate probes with novel properties.

Graphical abstract: A library of Rhodamine6G-based pH-sensitive fluorescent probes with versatile in vivo and in vitro applications

Supplementary files

Article information

Article type
Paper
Submitted
31 jan 2022
Accepted
24 apr 2022
First published
29 apr 2022
This article is Open Access
Creative Commons BY-NC license

RSC Chem. Biol., 2022,3, 748-764

A library of Rhodamine6G-based pH-sensitive fluorescent probes with versatile in vivo and in vitro applications

W. B. Swanson, M. Durdan, M. Eberle, S. Woodbury, A. Mauser, J. Gregory, B. Zhang, D. Niemann, J. Herremans, P. X. Ma, J. Lahann, M. Weivoda, Y. Mishina and C. F. Greineder, RSC Chem. Biol., 2022, 3, 748 DOI: 10.1039/D2CB00030J

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