Issue 21, 2022

Electrochemiluminescence biosensor for cardiac troponin I with signal amplification based on a MoS2@Cu2O–Ag-modified electrode and Ce:ZnO-NGQDs

Abstract

Cardiac troponin I (cTnI) is one of the structural subunits of cardiac troponin complexes and a significant biomarker of acute myocardial infarction (AMI). Therefore, a sensitive detection of cTnI in the early stages is of great significance. Here, a sensitive sandwiched electrochemiluminescence (ECL) immunosensor was built for the detection of cTnI, where molybdenum disulfide@cuprous oxide–silver nanoparticles (MoS2@Cu2O–Ag) immobilized cTnI the capture antibody (Ab1), and cerium-doped zinc oxide@nitrogen-doped graphene quantum dots (Ce:ZnO@NGQDs) loaded the signal antibody (Ab2). The MoS2@Cu2O–Ag nanoparticles not only indicated excellent electroconductivity and biocompatibility but also provided a large specific surface. Ce:ZnO as a co-reaction accelerator can convert the Ce4+ ↔ Ce3+ reaction and might increase the rate of electron exchange to accomplish signal enhancement. Under optimum conditions, the sensor possessed a wide linear range from 10 pg mL−1 to 100 ng mL−1, and the LOD was 2.90 fg mL−1 (S/N = 3). In addition, the sensor plays a good role in serum detection, indicating that it has potential for application in the detection of biomolecules.

Graphical abstract: Electrochemiluminescence biosensor for cardiac troponin I with signal amplification based on a MoS2@Cu2O–Ag-modified electrode and Ce:ZnO-NGQDs

Supplementary files

Article information

Article type
Paper
Submitted
15 aug 2022
Accepted
12 sep 2022
First published
13 sep 2022

Analyst, 2022,147, 4768-4776

Electrochemiluminescence biosensor for cardiac troponin I with signal amplification based on a MoS2@Cu2O–Ag-modified electrode and Ce:ZnO-NGQDs

B. Wang, C. Wang, Y. Li, X. Liu, D. Wu and Q. Wei, Analyst, 2022, 147, 4768 DOI: 10.1039/D2AN01341J

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