Issue 1, 2021

One-step enzyme-free dual electrochemical immunosensor for histidine-rich protein 2 determination

Abstract

In the present work, we describe a novel one-step enzyme-free dual electrochemical immunosensor for the determination of histidine-rich protein 2 (Ag-PfHRP2), a specific malaria biomarker. A gold electrode (GE) was functionalized with the PfHRP2 antibody (Ab-PfHRP2) using dihexadecyl phosphate (DHP) polymer as an immobilization platform. The Ab-PfHRP2/DHP/GE sensor was characterized by cyclic voltammetry, electrochemical impedance spectroscopy, Fourier-transform infrared spectroscopy, scanning electron microscopy, and atomic force microscopy. The developed immunosensor was employed for indirect Ag-PfHRP2 determination by differential pulse voltammetry (DPV) and electrochemical impedance spectroscopy (EIS). The linear range was 10–400 ng mL−1 and 10–500 ng mL−1 for EIS and DPV, while the limit of detection was 3.3 ng mL−1 and 2.8 ng mL−1, respectively. The electrochemical immunosensor was successfully applied for Ag-PfHRP2 determination in human serum samples. Its performance was compared with an ELISA test, and good correspondence was achieved. The coefficients of intra- and inter-assay variations were less than 5%. The electrochemical immunosensor is a useful and straightforward tool for in situ malaria biomarker determination.

Graphical abstract: One-step enzyme-free dual electrochemical immunosensor for histidine-rich protein 2 determination

Supplementary files

Article information

Article type
Paper
Submitted
13 okt 2020
Accepted
09 dec 2020
First published
23 dec 2020
This article is Open Access
Creative Commons BY license

RSC Adv., 2021,11, 408-415

One-step enzyme-free dual electrochemical immunosensor for histidine-rich protein 2 determination

A. M. Dip Gandarilla, M. Regiart, M. Bertotti, J. Correa Glória, L. A. Morais Mariuba and W. Ricardo Brito, RSC Adv., 2021, 11, 408 DOI: 10.1039/D0RA08729G

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