Issue 1, 2021

Self-assembled chromogen-loaded polymeric cocoon for respiratory virus detection

Abstract

Inspired by the self-assembly approach, in this work, the chromogen, 3,3′,5,5′-tetramethylbenzidine (TMB), was successfully co-precipitated in aqueous solution to form collective nanoparticles (NPs) of signal molecules (TMB-NPs). Utilizing poly(lactide-co-glycolide) (PLGA) in the molecular delivery approach, the formed emulsion nanovesicle (TMB-NPs@PLGA) exhibits an enrichment of the collective signal molecules in a single antibody–antigen conjugation. A specific antibody-conjugated TMB-NPs@PLGA forms an immunocomplex sandwich structure upon the addition of influenza virus (IV)/A. The addition of dimethyl sulfoxide (DMSO) dissolves the PLGA nanovesicles, releasing the encapsulated TMB-NPs. Sequentially, the TMB-NPs release TMB molecules upon the addition of DMSO. The released TMB is catalytically oxidized by H2O2 with self-assembled protein-inorganic nanoflowers, where copper nanoflowers (CuNFs) acted as the nanozyme. The developed immunoassay demonstrates high sensitivity for IV/A with a limit of detection (LOD) as low as 32.37 fg mL−1 and 54.97 fg mL−1 in buffer and serum, respectively. For practical needs, a clinically isolated IV/A/H3N2 and spike protein of SARS-CoV-2 were detected with the LODs of 17 pfu mL−1 and 143 fg mL−1, respectively. These results show the applicability of the advanced TMB-NPs@PLGA-based colorimetric sensor for the highly sensitive detection of airborne respiratory viruses.

Graphical abstract: Self-assembled chromogen-loaded polymeric cocoon for respiratory virus detection

Supplementary files

Article information

Article type
Paper
Submitted
26 sep 2020
Accepted
30 nov 2020
First published
08 dec 2020

Nanoscale, 2021,13, 388-396

Self-assembled chromogen-loaded polymeric cocoon for respiratory virus detection

I. M. Khoris, A. B. Ganganboina, T. Suzuki and E. Y. Park, Nanoscale, 2021, 13, 388 DOI: 10.1039/D0NR06893D

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