Issue 3, 2021

Recent advances in the use of stimulated Raman scattering in histopathology

Abstract

Stimulated Raman histopathology (SRH) utilises the intrinsic vibrational properties of lipids, proteins and nucleic acids to generate contrast providing rapid image acquisition that allows visualisation of histopathological features. It is currently being trialled in the intraoperative setting, where the ability to image unprocessed samples rapidly and with high resolution offers several potential advantages over the use of conventional haematoxylin and eosin stained images. Here we review recent advances in the field including new updates in instrumentation and computer aided diagnosis. We also discuss how other non-linear modalities can be used to provide additional diagnostic contrast which together pave the way for enhanced histopathology and open up possibilities for in vivo pathology.

Graphical abstract: Recent advances in the use of stimulated Raman scattering in histopathology

Article information

Article type
Minireview
Submitted
05 okt 2020
Accepted
15 dec 2020
First published
04 jan 2021
This article is Open Access
Creative Commons BY-NC license

Analyst, 2021,146, 789-802

Recent advances in the use of stimulated Raman scattering in histopathology

M. Lee, C. S. Herrington, M. Ravindra, K. Sepp, A. Davies, A. N. Hulme and V. G. Brunton, Analyst, 2021, 146, 789 DOI: 10.1039/D0AN01972K

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