Issue 8, 2019

Simultaneous dual-colour tracking lipid droplets and lysosomes dynamics using a fluorescent probe

Abstract

After entering a cell, most small molecule fluorescent probes are dispersed in the cytoplasm before they then accumulate in a specific organelle or subcellular zone. Molecules that can enter two or more organelles with high selectivity are all but unknown. In this work, we report a naphthalimide-based fluorescent probe, NIM-7, that allows lipid droplets and lysosomes to be labelled simultaneously and with high specificity. These subcellular entities can then be visualized readily through yellow and red fluorescence, using different excitation and detection channels. NIM-7 allows 3D imaging and quantitative visualizing of lipid droplets and lysosomes. It is also able to track simultaneously the movement of lipid droplets and lysosomes in real-time. We also report here that NIM-7 can be used to image both different cell lines and zebrafish embryos.

Graphical abstract: Simultaneous dual-colour tracking lipid droplets and lysosomes dynamics using a fluorescent probe

Supplementary files

Article information

Article type
Edge Article
Submitted
08 okt 2018
Accepted
21 dec 2018
First published
27 dec 2018
This article is Open Access

All publication charges for this article have been paid for by the Royal Society of Chemistry
Creative Commons BY-NC license

Chem. Sci., 2019,10, 2342-2348

Simultaneous dual-colour tracking lipid droplets and lysosomes dynamics using a fluorescent probe

X. Zheng, W. Zhu, F. Ni, H. Ai, S. Gong, X. Zhou, Jonathan L. Sessler and C. Yang, Chem. Sci., 2019, 10, 2342 DOI: 10.1039/C8SC04462G

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