Issue 4, 2019

Detection of leucine aminopeptidase activity in serum using surface-enhanced Raman spectroscopy

Abstract

Leucine aminopeptidase (LAP), an important proteolytic enzyme, is closely associated with diverse physiological and pathological disorders such as liver injury and cancers. Hence, it is imperative to develop an effective method to detect LAP activity for early diagnosis of diseases. In this work, we report a novel SERS probe bis-s-s′-[(s)-2-amino-N-(3-thiophenyl)-Leu]. (b-(s)-ANT-Leu) with an L-leucine amide group, which can specially respond to LAP, to assay the LAP activity according to the SERS spectral changes between the probe molecule and its corresponding hydrolysis product resulting from the catalysis of LAP. This SERS approach features high selectivity on account of the specificity of the reaction combined with the instinctive fingerprinting ability of SERS and shows a good linear relationship in a wide range from 0.2 to 100 mU mL−1 with a detection limit as low as 0.16 mU mL−1. In addition, the SERS-based strategy can be competent for LAP activity detection in clinical patient serum samples and LAP inhibitor evaluation, demonstrating its great potential in the pathological analysis for diseases involving LAP and the screening of LAP inhibitors.

Graphical abstract: Detection of leucine aminopeptidase activity in serum using surface-enhanced Raman spectroscopy

Supplementary files

Article information

Article type
Paper
Submitted
12 nov 2018
Accepted
10 dec 2018
First published
11 dec 2018

Analyst, 2019,144, 1394-1400

Detection of leucine aminopeptidase activity in serum using surface-enhanced Raman spectroscopy

D. Guo, Z. Gan, L. Jiang, M. Cao, F. T. Patrice, M. E. Hafez and D. Li, Analyst, 2019, 144, 1394 DOI: 10.1039/C8AN02182A

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements