Issue 18, 2018

High-throughput single-molecule bioassay using micro-reactor arrays with a concentration gradient of target molecules

Abstract

Micro-reactor arrays enable highly sensitive and quantitative bioassays at a single-molecule level. Accordingly, they are widely used for sensitive “digital” bioassays, e.g., digital PCR and digital ELISA. Despite high integration, individual reactors in digital bioassays are filled with a uniform reaction solution, thus limiting the ability to simultaneously conduct multiple bioassays under different conditions using integrated reactors in parallel, resulting in the loss of potential throughput. We developed micro-reactor arrays with a concentration gradient of target molecules, in which individual reactors sealed with a lipid-bilayer membrane contained a precise amount of target molecules. Using the arrays, we successfully demonstrated multiple single-molecule bioassays in parallel using alkaline phosphatase or α-hemolysin, key components in various biomedical sensors. This new platform extends the versatility of micro-reactor arrays and could enable further analytical and pharmacological applications.

Graphical abstract: High-throughput single-molecule bioassay using micro-reactor arrays with a concentration gradient of target molecules

Supplementary files

Article information

Article type
Paper
Submitted
28 mei 2018
Accepted
29 jun 2018
First published
09 aug 2018
This article is Open Access
Creative Commons BY license

Lab Chip, 2018,18, 2849-2853

High-throughput single-molecule bioassay using micro-reactor arrays with a concentration gradient of target molecules

R. Watanabe, T. Komatsu, S. Sakamoto, Y. Urano and H. Noji, Lab Chip, 2018, 18, 2849 DOI: 10.1039/C8LC00535D

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. You can use material from this article in other publications without requesting further permissions from the RSC, provided that the correct acknowledgement is given.

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