Issue 18, 2018

Monitoring real-time hormone release kinetics via high-content 3-D imaging of compensatory endocytosis

Abstract

High-content real-time imaging of hormone secretion in tissues or cell populations is a challenging task, which is unlikely to be resolved directly, despite immense translational value. We approach this problem indirectly, using compensatory endocytosis, a process that closely follows exocytosis in the cell, as a surrogate read-out for secretion. The tissue is immobilized in an open-air perifusion chamber and imaged using a two-photon microscope. A fluorescent polar tracer, perifused through the experimental circuit, gets trapped into the cells via endocytosis, and is quantified using a feature-detection algorithm. The signal of the tracer that accumulates into the endocytotic system reliably reflects stimulated exocytosis, which is demonstrated via co-imaging of the latter using existing reporters. A high signal-to-noise ratio and compatibility with multisensor imaging affords the real-time quantification of the secretion at the tissue/population level, whereas the cumulative nature of the signal allows imprinting of the “secretory history” within each cell. The technology works for several cell types, reflects disease progression and can be used for human tissue.

Graphical abstract: Monitoring real-time hormone release kinetics via high-content 3-D imaging of compensatory endocytosis

Supplementary files

Article information

Article type
Paper
Submitted
20 apr 2018
Accepted
26 jul 2018
First published
30 jul 2018
This article is Open Access
Creative Commons BY license

Lab Chip, 2018,18, 2838-2848

Monitoring real-time hormone release kinetics via high-content 3-D imaging of compensatory endocytosis

A. I. Tarasov, J. Galvanovskis, O. Rorsman, A. Hamilton, E. Vergari, P. R. V. Johnson, F. Reimann, F. M. Ashcroft and P. Rorsman, Lab Chip, 2018, 18, 2838 DOI: 10.1039/C8LC00417J

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