Issue 6, 2017

Reaction-based indicator displacement assay (RIA) for the colorimetric and fluorometric detection of hydrogen peroxide

Abstract

Based on the complexation of phenylboronic acid (PBA) with Alizarin Red S (ARS), we developed a new chemosensor for the detection of hydrogen peroxide (H2O2) in aqueous media. This platform has demonstrated its ability to detect H2O2via colorimetric, fluorometric, and electrochemical measurements. The experimental observations reveal that the system displays a red-shifted visible colour change, on–off fluorescence response indicating release of indicator (ARS) and turn-on electrochemical signal indicating generation of phenol, after reaction with H2O2. With this work we have demonstrated that our reaction-based indicator displacement assay (RIA) systems, can be employed as an assay for H2O2 and hydrogen peroxide-related species for environmental and physiological detection.

Graphical abstract: Reaction-based indicator displacement assay (RIA) for the colorimetric and fluorometric detection of hydrogen peroxide

Supplementary files

Article information

Article type
Research Article
Submitted
08 aug 2016
Accepted
13 feb 2017
First published
17 feb 2017
This article is Open Access
Creative Commons BY license

Org. Chem. Front., 2017,4, 1058-1062

Reaction-based indicator displacement assay (RIA) for the colorimetric and fluorometric detection of hydrogen peroxide

X. Sun, M. L. Odyniec, A. C. Sedgwick, K. Lacina, S. Xu, T. Qiang, S. D. Bull, F. Marken and T. D. James, Org. Chem. Front., 2017, 4, 1058 DOI: 10.1039/C6QO00448B

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