Issue 2, 2017

Transaminase biocatalysis: optimization and application

Abstract

Transaminases (TAs) are one of the most promising biocatalysts in organic synthesis for the preparation of chiral amino compounds. The concise reaction, excellent enantioselectivity, environmental friendliness and compatibility with other enzymatic or chemical systems have brought TAs to the attention of scientists working in the area of biocatalysis. However, to utilize TAs in a more efficient and economical way, attempts have to be made to optimize their performance. The demand for various substrate specificities, stability under non-physiological conditions and higher conversions in reversible reactions have been targeted and investigated thoroughly. A number of both protein- and process-based strategies have been developed to improve TAs and systems involving TAs. Moreover, by combination with other enzymes in cascade reactions or even in more complex systems, so called synthetic biology and systems biocatalysis, TAs can be biocatalysts with immense potential in the industrial production of high-value chemical products. This review will highlight strategies for optimization of TAs and will discuss a number of elegant systems for improving their performance. Transaminase biocatalysis has been, and will continue to be, one of the most interesting topics in green organic synthesis.

Graphical abstract: Transaminase biocatalysis: optimization and application

Article information

Article type
Critical Review
Submitted
20 aug 2016
Accepted
30 sep 2016
First published
30 sep 2016
This article is Open Access
Creative Commons BY license

Green Chem., 2017,19, 333-360

Transaminase biocatalysis: optimization and application

F. Guo and P. Berglund, Green Chem., 2017, 19, 333 DOI: 10.1039/C6GC02328B

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. You can use material from this article in other publications without requesting further permissions from the RSC, provided that the correct acknowledgement is given.

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