Issue 94, 2017

A continuous luminescence assay for monitoring kinase activity: signalling the ADP/ATP ratio using a discrete europium complex

Abstract

We report the application of a stable cationic europium complex [Eu.1]+ in a continuous-read luminescence assay for kinase activity. [Eu.1]+ binds reversibly to ATP and ADP in water, at neutral pH, in the presence of Mg2+ ions, providing distinctive luminescence responses that permits the kinase-catalysed conversion of ATP to ADP to be monitored in real-time.

Graphical abstract: A continuous luminescence assay for monitoring kinase activity: signalling the ADP/ATP ratio using a discrete europium complex

Supplementary files

Article information

Article type
Communication
Submitted
28 jul 2017
Accepted
22 sep 2017
First published
13 nov 2017
This article is Open Access
Creative Commons BY license

Chem. Commun., 2017,53, 12626-12629

A continuous luminescence assay for monitoring kinase activity: signalling the ADP/ATP ratio using a discrete europium complex

S. H. Hewitt, J. Parris, R. Mailhot and S. J. Butler, Chem. Commun., 2017, 53, 12626 DOI: 10.1039/C7CC05887J

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