Issue 59, 2015

Rapid separation and on-line detection by coupling high performance liquid chromatography with surface-enhanced Raman spectroscopy

Abstract

Rapid separation and detection of analytes have been the focus of a growing body of investigation for potential applications including food safety and environment science. However, the development of a robust analytical technique for simultaneous rapid separation and on-line detection remains a formidable challenge. Herein, we report a rational design based on the combination of high performance liquid chromatography (HPLC) and surface-enhanced Raman spectroscopy (SERS) for the rapid separation and on-line detection of multi-analytes. In particular, a plasmonic nanoparticle-modified capillary (NPMC) is fabricated through a self-assembly process and connected to a HPLC effluent-end port. After separation by HPLC, the analytes are adsorbed onto plasmonic nanoparticles in the capillary and then detected by SERS. The resulting HPLC-SERS coupled detection system can simultaneously achieve rapid separation and provide on-line molecular structural information of multi-analytes. In addition, we also demonstrate the on-line detection of a pesticide molecule (thiram) in an orange using this combined system. Importantly, the detection limit can be down to 10āˆ’7 mol Lāˆ’1. These findings indicate that our coupled HPLC-SERS system offers a promising analytical technique in modern analytical science and technology.

Graphical abstract: Rapid separation and on-line detection by coupling high performance liquid chromatography with surface-enhanced Raman spectroscopy

Supplementary files

Article information

Article type
Paper
Submitted
29 mrt 2015
Accepted
08 mei 2015
First published
12 mei 2015

RSC Adv., 2015,5, 47640-47646

Rapid separation and on-line detection by coupling high performance liquid chromatography with surface-enhanced Raman spectroscopy

W. Wang, M. Xu, Q. Guo, Y. Yuan, R. Gu and J. Yao, RSC Adv., 2015, 5, 47640 DOI: 10.1039/C5RA05562H

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements