Issue 12, 2015

Expanding the toolbox of organic chemists: directed evolution of P450 monooxygenases as catalysts in regio- and stereoselective oxidative hydroxylation

Abstract

Cytochrome P450 enzymes (CYPs) have been used for more than six decades as catalysts for the CH-activating oxidative hydroxylation of organic compounds with formation of added-value products. However, it has not been possible to control regio- and stereoselectivity in a general manner, which is necessary for wide (industrial) applications. Especially directed evolution as a Darwinian approach to protein engineering has changed the situation for the better during the last 3–4 years leading to extensive progress, which is summarized and analysed in this Feature Article.

Graphical abstract: Expanding the toolbox of organic chemists: directed evolution of P450 monooxygenases as catalysts in regio- and stereoselective oxidative hydroxylation

Article information

Article type
Feature Article
Submitted
18 nov 2014
Accepted
28 nov 2014
First published
08 dec 2014

Chem. Commun., 2015,51, 2208-2224

Author version available

Expanding the toolbox of organic chemists: directed evolution of P450 monooxygenases as catalysts in regio- and stereoselective oxidative hydroxylation

G. Roiban and M. T. Reetz, Chem. Commun., 2015, 51, 2208 DOI: 10.1039/C4CC09218J

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