Issue 10, 2021

Engineering of biofilms with a glycosylation circuit for biomaterial applications

Abstract

Glycosylation is a crucial post-translational modification for a wide range of functionalities. Adhesive protein-based biomaterials in nature rely on heavily glycosylated proteins such as spider silk and mussel adhesive proteins. Engineering protein-based biomaterials genetically enables desired functions and characteristics. Additionally, utilization of glycosylation for biomaterial engineering can expand possibilities by including saccharides to the inventory of building blocks. Here, de novo glycosylation of Bacillus subtilis amyloid-like biofilm protein TasA using a Campylobacter jejuni glycosylation circuit is proposed to be a novel biomaterial engineering method for increasing adhesiveness of TasA fibrils. A C. jejuni glycosylation motif is genetically incorporated to tasA gene and expressed in Escherichia coli containing the C. jejuni pgl protein glycosylation pathway. Glycosylated TasA fibrils indicate enhanced adsorption on the gold surface without disruption of fibril formation. Our findings suggest that N-linked glycosylation can be a promising tool for engineering protein-based biomaterials specifically regarding adhesion.

Graphical abstract: Engineering of biofilms with a glycosylation circuit for biomaterial applications

Supplementary files

Article information

Article type
Paper
Submitted
25 dec 2020
Accepted
14 feb 2021
First published
19 feb 2021

Biomater. Sci., 2021,9, 3650-3661

Engineering of biofilms with a glycosylation circuit for biomaterial applications

E. Sahin Kehribar, M. E. Isilak, E. U. Bozkurt, J. Adamcik, R. Mezzenga and U. O. S. Seker, Biomater. Sci., 2021, 9, 3650 DOI: 10.1039/D0BM02192J

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements