Issue 2, 2017

Design of recombinant whole-cell catalysts for double reduction of C[double bond, length as m-dash]C and C[double bond, length as m-dash]O bonds in enals and application in the synthesis of Guerbet alcohols as industrial bulk chemicals for lubricants

Abstract

Whole-cell catalysts overexpressing two enzymes for a double reduction cascade in which aliphatic α-branched α,β-unsaturated aldehydes are converted into Guerbet alcohols as a highly demanded class of lubricants were constructed and characterized. The reaction conditions were optimized to suppress by-product formation, achieving high conversion and selectivity. Application of methanol as a co-solvent on a 500 mL scale enabled a substrate concentration of 18.5 g L−1 obtaining 97% yield and 88% purity without further distillation. Addressing the needs of industrial processes, substrate loading was increased up to 94 g L−1 and the E-factor (environmental factor) was significantly improved compared to the original process with isolated enzymes.

Graphical abstract: Design of recombinant whole-cell catalysts for double reduction of C [[double bond, length as m-dash]] C and C [[double bond, length as m-dash]] O bonds in enals and application in the synthesis of Guerbet alcohols as industrial bulk chemicals for lubricants

Supplementary files

Article information

Article type
Paper
Submitted
18 jun 2016
Accepted
08 aug 2016
First published
06 sep 2016

Green Chem., 2017,19, 405-410

Design of recombinant whole-cell catalysts for double reduction of C[double bond, length as m-dash]C and C[double bond, length as m-dash]O bonds in enals and application in the synthesis of Guerbet alcohols as industrial bulk chemicals for lubricants

M. Biermann, D. Bakonyi, W. Hummel and H. Gröger, Green Chem., 2017, 19, 405 DOI: 10.1039/C6GC01668E

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