Issue 5, 2010

Vancomycin dimer formation between analogues of bacterial peptidoglycan surfaces probed by force spectroscopy

Abstract

Functionalised thiols presenting peptides found in the peptidoglycan of vancomycin-sensitive and -resistant bacteria were synthesised and used to form self-assembled monolayers (SAMs) on gold surfaces. This model bacterial cell-wall surface mimic was used to study binding interactions with vancomycin. Force spectroscopy, using the atomic force microscope (AFM), was used to investigate the specific rupture of interfacial vancomycin dimer complexes formed between pairs of vancomycin molecules bound to peptide-coated AFM probe and substrate surfaces. Clear adhesive contacts were observed between the vancomycin-sensitive peptide surfaces when vancomycin was present in solution, and the adhesion force demonstrated a clear dependence on antibiotic concentration.

Graphical abstract: Vancomycin dimer formation between analogues of bacterial peptidoglycan surfaces probed by force spectroscopy

Supplementary files

Article information

Article type
Paper
Submitted
17 sep 2009
Accepted
26 nov 2009
First published
08 jan 2010

Org. Biomol. Chem., 2010,8, 1142-1148

Vancomycin dimer formation between analogues of bacterial peptidoglycan surfaces probed by force spectroscopy

M. Batchelor, D. Zhou, M. A. Cooper, C. Abell and T. Rayment, Org. Biomol. Chem., 2010, 8, 1142 DOI: 10.1039/B919347B

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