Issue 4, 2003

Sub-second isoelectric focusing in free flow using a microfluidic device

Abstract

Using a microfabricated chip with a bed volume of 0.2 µL we demonstrate the validity of the scaling laws for molecular mass transport of isoelectric focusing (IEF) in free flow. Nano- or microlitre sample volumes can be concentrated within 430 ms by a factor of up to 400. These very fast performances make the chip applicable to proteomic analysis and for continuous monitoring of biochemical processes.

Article information

Article type
Communication
Submitted
22 jul 2003
Accepted
17 sep 2003
First published
07 okt 2003

Lab Chip, 2003,3, 224-227

Sub-second isoelectric focusing in free flow using a microfluidic device

Y. Xu, C. Zhang, D. Janasek and A. Manz, Lab Chip, 2003, 3, 224 DOI: 10.1039/B308476K

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