This review summarises the use of engineered ketoreductases (KREDS), both as whole microbial cells and isolated enzymes, in the highly enantiospecific reduction of prochiral ketones.
An asymmetric sustainable route to D-pantothenic acid was developed, centering on the reduction of ethyl 2′-ketopantothenate to ethyl (R)-pantothenate, enabled by an E. coli strain co-expressing an engineered ketoreductase and glucose dehydrogenase.
Automated high throughput workflow facilitating the rapid optimization and development of immobilized enzymes – ready to be scaled – and thus matching the need for speed in modern drug development.
The synthesis of enantiopure chiral halogenated aryl alcohols by ketoreductases was conducted in an aqueous micellar solution formed by TPGS-750-M, achieving remarkable yields and enantioselectivities under high concentration.
In this review article, recent developments and applications of microbial alcohol dehydrogenases are summarized by emphasizing notable examples.