A high-speed sequential liquid compartmentalization method for digital loop-mediated isothermal amplification in a microfluidic device

Riku Honda, Taketo Saruwatari, Daigo Natsuhara, Yuka Kiba, Shunya Okamoto, Moeto Nagai, Masashi Kitamura and Takayuki Shibata

Lab Chip, 2025, 25, 4787
DOI: 10.1039/D5LC00486A

Microfluidic encapsulation of DNAs in liquid beads for digital PCR application

Fariba Malekpour Galogahi, Melody Christie, Ajeet Singh Yadav, Hongjie An, Helen Stratton and Nam-Trung Nguyen

Analyst, 2023, 148, 4064
DOI: 10.1039/D3AN00868A

Lightweight target detection for large-field ddPCR images based on improved YOLOv5

Xingyu Jin, Jing Yang, Xiaorui Jiang, Zhenqing Li, Jinrong Shen, Zhiheng Yu, Cunliang Yang, Fengli Huang, Dunlu Peng, Yoshinori Yamaguchi and Jijun Feng

Digital Discovery, 2025, 4, 1298
DOI: 10.1039/D5DD00006H

Droplets in open microfluidics: generation, manipulation, and application in cell analysis

Jiaxu Lin, Ying Hou, Qiang Zhang and Jin-Ming Lin

Lab Chip, 2025, 25, 787
DOI: 10.1039/D4LC00646A

Multiple on-line active valves based centrifugal microfluidics for dynamic solid-phase enrichment and purification of viral nucleic acid

Shunji Li, Chao Wan, Yujin Xiao, Changgen Liu, Xudong Zhao, Ying Zhang, Huijuan Yuan, Liqiang Wu, Chungen Qian, Yiwei Li, Peng Chen and Bi-Feng Liu

Lab Chip, 2024, 24, 3158
DOI: 10.1039/D4LC00074A

Digital PCR: from early developments to its future application in clinics

Amandine Trouchet, Guillaume Gines, Leonor Benhaim and Valerie Taly

Lab Chip, 2025, 25, 3921
DOI: 10.1039/D5LC00055F

Reusable EWOD-based microfluidic system for active droplet generation

Suhee Park, Jaewook Ryu and Ki-Ho Han

Lab Chip, 2025, 25, 225
DOI: 10.1039/D4LC00744A

Development and future of droplet microfluidics

Lang Nan, Huidan Zhang, David A. Weitz and Ho Cheung Shum

Lab Chip, 2024, 24, 1135
DOI: 10.1039/D3LC00729D

Development of a non-transfer microdroplet generation chip and digital molecular detection system

Rui Sun, Lei Li, Tong Fang, Chaoyi Hu, Haiwen Li and Huancai Yin

Anal. Methods, 2025, 17, 4796
DOI: 10.1039/D5AY00377F

A capillary-based centrifugal indicator equipped with in situ pathogenic bacteria culture for fast antimicrobial susceptibility testing

Longyu Chen, Meijia Zhu, Zhiyong Wang, Hongliang Wang, Yongqiang Cheng, Ziwei Zhang, Xiaoxiao Qi, Yifan Shao, Xi Zhang and Hongwei Wang

Analyst, 2024, 149, 2420
DOI: 10.1039/D3AN02144K

Digital PCR in noninvasive prenatal testing: Analytical principles, clinical utilities, and future integration

Wen Zeng, Wenbo Luo, Junwei Zhou, Bohang Wang, Hao Qin, Yang He, Imrich Gablech, Marie Korabečná and Pavel Neužil

TrAC Trends in Analytical Chemistry, 2026, 197, 118695
DOI: 10.1016/j.trac.2026.118695

Photothermally Driven Ultrafast Polymerase Chain Reaction: Mechanisms, Nanomaterial Architectures, and System Integration

Yile Fang, Lijun Cai, Ning Li, Feika Bian, Dagan Zhang, Nongyue He, Zhiyang Li, Hong Yan and Yuanjin Zhao

Research, 2025, 8
DOI: 10.34133/research.0839

Fabrication and target applications of hydrophilic-hydrophobic composite wettability surfaces based on surface wettability gradient and Laplace pressure gradient regulation

Dawei Luo, Jun Zhang, Xinjuan Zeng, Min Zhang, Xinan Zeng and Cailong Zhou

Applied Materials Today, 2023, 35, 101957
DOI: 10.1016/j.apmt.2023.101957

Morphology evolution and dynamics of sliding nanodroplets under external forces: A molecular dynamics study

Wenbin Liu, Jianguo Zhang and Hong Liu

Physics of Fluids, 2023, 35
DOI: 10.1063/5.0157835

From droplets to diagnosis: AI-driven imaging and system integration in digital nucleic acid amplification testing

Yuanyuan Wei, Xianxian Liu, Yao Mu, Changran Xu, Guoxun Zhang, Tianhao Li, Zida Li, Wu Yuan, Ho-Pui Ho and Mingkun Xu

Biosensors and Bioelectronics, 2025, 288, 117741
DOI: 10.1016/j.bios.2025.117741

Multi-factor coupled thermal simulation of flat-panel digital PCR structure

Qixuan Li, Xulei Qin and Haoyu Wang

J. meas. eng., 2024, 12, 214
DOI: 10.21595/jme.2024.23599

A Poisson-Independent Approach to Precision Nucleic Acid Quantification in Microdroplets

Reya Ganguly and Chang-Soo Lee

ACS Appl. Bio Mater., 2024, 7, 3441
DOI: 10.1021/acsabm.4c00350

An approach for integrating droplet generation and detection in digital polymerase chain reaction applications based on a bifunctional microfluidic cross-structure

Jinxian Wang, Xin Lyu, Xiaoliang Zhang, Shun Wang, Wen Zeng, Tianhang Yang, Bidou Wang and Gangyin Luo

Talanta, 2024, 267, 125240
DOI: 10.1016/j.talanta.2023.125240

Construction of a monoclonal molecular imprinted sensor with high affinity for specific recognition of influenza a virus subtype

Hang Gong, Ganping Cai, Chunyan Chen, Feng Chen and Changqun Cai

Talanta, 2024, 278, 126568
DOI: 10.1016/j.talanta.2024.126568

Peptide Nucleic Acid Clamp‐Assisted Photothermal Multiplexed Digital PCR for Identifying SARS‐CoV‐2 Variants of Concern

Lexiang Zhang, Rokshana Parvin, Siyue Lin, Mingshuo Chen, Ruixuan Zheng, Qihui Fan and Fangfu Ye

Advanced Science, 2024, 11
DOI: 10.1002/advs.202306088

A chip-based universal strategy to realize multiplex PCR by using wax films for sealing and controllable release of primers

Nan Li, Yuanyue Zhang, Huili Wang, Xun Xu, Xiaoye Huo, Junbo Wang and Youchun Xu

Biosensors and Bioelectronics, 2025, 269, 116921
DOI: 10.1016/j.bios.2024.116921

Recent advancements in single-cell metabolic analysis for pharmacological research

Ying Hou, Hongren Yao and Jin-Ming Lin

Journal of Pharmaceutical Analysis, 2023, 13, 1102
DOI: 10.1016/j.jpha.2023.08.014

A State‐Of‐The‐Art Review of Aquatic eDNA Sampling Technologies and Instrumentation: Advancements, Challenges, and Future Prospects

Kevan M. Yamahara, Elizabeth A. Allan, Julie Robidart, William H. Wilson, James M. Birch, Pascal Craw, Ethan Edson, Ivory B. Engstrom, Tatsuhiro Fukuba, Annette F. Govindarajan, Alfredo M. Martins, Kim M. Parsons, Vincent J. Sieben, Austen Thomas, Ian Wilson and Christopher A. Scholin

Environmental DNA, 2025, 7
DOI: 10.1002/edn3.70170

Sequential Flow Controllable Microfluidic Device for G-Quadruplex DNAzyme-Based Electrochemical Detection of SARS-CoV-2 Using a Pyrrolidinyl Peptide Nucleic Acid

Sarida Naorungroj, Chawin Srisomwat, Wisarut Khamcharoen, Sakda Jampasa, Ekawat Pasomsub, Kwanwoo Shin, Tirayut Vilaivan and Orawon Chailapakul

Anal. Chem., 2023, 95, 12794
DOI: 10.1021/acs.analchem.3c01758

Monolayer-fluorescence counting for ultrasensitive detection of tumour cell-derived extracellular vesicles using a step-wedge microfluidic platform

Chang-Xin Ji, Meng-Fan Zhang, Xuan Wang, Huihong Liu, Mei-Hui Wu, Wei Li, Zi-Li Yu and Shao-Li Hong

Sensors and Actuators B: Chemical, 2025, 423, 136786
DOI: 10.1016/j.snb.2024.136786

Absolute Quantitative Detection of Hypervirulent Klebsiella pneumoniae Using a Novel Silicon-Doped Carbon Quantum Dot-Probe-Graphene Oxide

Lijuan Huang, Bing Du, Xiaohu Cui, Meiqi Liu, Zhoufei Li, Xiaoyun Cui, Ziying Xu, Zihui Yu, Shuo Zhao, Yanling Feng, Hanqing Zhao, Jianhai Long and Jing Yuan

ACS Sens., 2025, 10, 5633
DOI: 10.1021/acssensors.5c00508

Pico-Scale Digital PCR on a Super-Hydrophilic Microarray Chip for Multi-Target Detection

Qingyue Xian, Jie Zhang, Yu Ching Wong, Yibo Gao, Qi Song, Na Xu and Weijia Wen

Micromachines, 2025, 16, 407
DOI: 10.3390/mi16040407

Development and validation of a duplex droplet digital PCR assay for the simultaneous detection of cytomegalovirus and Epstein-Barr virus in plasma

Zhaoxiang Du, Xingxing Yuan, Sihan Zhou, Lili Zhang, Yifeng Wang, Jie Yi, Manyu Li, Yan Dang, Ning Liu, Xin Liu, Fangfang Dai, Haiqing Sun, Yanhua Yu and Gengxia Yang

Front. Cell. Infect. Microbiol., 2026, 16
DOI: 10.3389/fcimb.2026.1798127

Bioinspired Surfaces Derived from Acoustic Waves for On-Demand Droplet Manipulations

Zhuhao Wu, Lingyu Sun, Hanxu Chen and Yuanjin Zhao

Research, 2023, 6
DOI: 10.34133/research.0263

Droplet digital RT-PCR method for SARS-CoV-2 variants detection in clinical and wastewater samples

Feng Wang, Yi Sun, Liming Gong, Lingxuan Su, Biaofeng Zhou, Xiuyu Lou, Yin Chen, Wen Shi, Haiyan Mao and Yanjun Zhang

Front. Microbiol., 2025, 16
DOI: 10.3389/fmicb.2025.1635733

Droplet Microfluidics: From Generation to Manipulation

Nan Xiang, Lin Jiang and Zhonghua Ni

Micromachines, 2025, 16, 1359
DOI: 10.3390/mi16121359

Dynamic Interfacial Printing Driven by Galvanometer Scanner for Monodisperse Single and Double Emulsion Droplets

Qichen He, Guanghui Gao, Shenglong Liao and Shouchun Yin

Part & Part Syst Charact, 2025, 42
DOI: 10.1002/ppsc.202400290

Ultrasensitive quantification of aflatoxin B1 via nanobody phage -mediated quantitative PCR and digital PCR for agro-products

Junjie Zeng, Yanping Li, Qinghua He, Ji Xia, Jiebiao Guo and Zhui Tu

Biosensors and Bioelectronics, 2025, 286, 117622
DOI: 10.1016/j.bios.2025.117622

Massive and controllable production of droplets on chips induced by surface acoustic waves for multiplex digital PCR detection of bacteria

Guangyang Li, Ziqiao Zhou, Xuefeng Xu, Xi Yang, Mingyu Cui, Zhi Ruan, Shengjun Wu, Shurong Dong, Jikui Luo and Zhen Cao

Sensors and Actuators B: Chemical, 2026, 446, 138763
DOI: 10.1016/j.snb.2025.138763

Development of droplet-based microfluidics in generation, manipulation and biomedical application

Yi Cai, Runze Sun, Yukun Ren and Yixing Gou

Microchemical Journal, 2025, 211, 113101
DOI: 10.1016/j.microc.2025.113101

An Electrochemical Sensor Based on AuNPs@Cu-MOF/MWCNTs Integrated Microfluidic Device for Selective Monitoring of Hydroxychloroquine in Human Serum

Xuanlin Feng, Jiaqi Zhao, Shiwei Wu, Ying Kan, Honemei Li and Weifei Zhang

Chemosensors, 2025, 13, 200
DOI: 10.3390/chemosensors13060200

Self-service multimodal detection of subtype influenza A virus H5N1 by visual portable molecular imprinting sensor

Hang Gong, Li Tang, Feng Chen, Chunyan Chen, Yi Cheng and Changqun Cai

Chemical Engineering Journal, 2024, 483, 148946
DOI: 10.1016/j.cej.2024.148946

An integrated droplet microfluidic chip for digital polymerase chain reaction with a fountain-like structure capable of removing bubble

Keming Jiang, Tao Zhang, Cong Liu, Zhiqiang Zhang and Haiwen Li

Sensors and Actuators B: Chemical, 2025, 433, 137540
DOI: 10.1016/j.snb.2025.137540

Advancements and Future Perspectives of Microfluidic Technology in Pediatric Healthcare

Xuting Zhang, Andong Liu, Yanke Wang, Chao Niu, Xing Rong, Chang Jia, Jia Sun, Shiyang Song, Lexiang Zhang, Fangfu Ye, Changmin Shao and Maoping Chu

Smart Medicine, 2025, 4
DOI: 10.1002/smmd.70018

Magnetic-based Microfluidic Chip: A Powerful Tool for Pathogen Detection and Affinity Reagents Selection

Shao-Li Hong, Meng-Fan Zhang, Xuan Wang, Huihong Liu, Nangang Zhang, Man Tang and Wei Li

Critical Reviews in Analytical Chemistry, 2024, 54, 2658
DOI: 10.1080/10408347.2023.2195940

Navigating the molecular landscape: integrated multiomics liquid biopsy for biomarker discovery in early detection and monitoring of colorectal cancer

Xuanqiang Fan, Jinyu Shi, Yiwan Shang and Hui Xu

Front. Mol. Biosci., 2026, 13
DOI: 10.3389/fmolb.2026.1795133

A high-throughput droplet digital PCR system aiming eight DNA methylation targets for age prediction

Yuxiang Zhou, Yanyun Wang, Mengyuan Song, Lanrui Jiang, Chaoran Sun, Shuangshuang Wang, Hewen Yao, Zefei Wang, Xindi Wang, Chunhui Liu, Haibo Luo and Feng Song

Journal of Pharmaceutical and Biomedical Analysis, 2024, 240, 115943
DOI: 10.1016/j.jpba.2023.115943

A novel microfluidic colorimetric biosensor for rapid and automatic detection Escherichia coli O157:H7 in aquaponics water

Zhuangzhuang Bai, Bingxiong Wang, Tingyao Gao, Xianbao Xu, Zhuangzhuang Du, Jie Han, Yang Hu, Yu Bai, Liu Wang, Cong Wang and Daoliang Li

Computers and Electronics in Agriculture, 2025, 229, 109941
DOI: 10.1016/j.compag.2025.109941

Development and Evaluation of a Droplet Digital PCR Assay for the Accurately Detecting the CircHIPK3 in Plasma Samples from Patients with Hepatocellular Carcinoma

Yuanye Ji, Ping Tuo, Shun Zhang, Ting Cai, Liyun Fu, Qinzhi Deng, Houdao Fu, Guosheng Gao, Fajiu Wang and Peng Zhu

J. Microbiol. Biotechnol., 2025, 35
DOI: 10.4014/jmb.2412.12048

Accurate and Microfluidics-Free Digital Nucleic Acid Quantification with Crescent Microbeads-Templated Emulsions

Long Chen, Yanan Du, Sixiang Rao, Qingyuan Shi, Yuchen Cui, Lele Zhou, Chengwei Xiong, Yuan Luo and Yifan Liu

ACS Sens., 2025, 10, 6645
DOI: 10.1021/acssensors.5c01158

Lab-on-a-chip technologies: The future of precision medicine and high-throughput biomolecular analysis

Tianfeng Xu, Jie Hu, Chongyu Liu, Hao Bai, Limei Zhang, Weihua Zhuang, Gang Wang, Zhongyang Li, Haichen Lv, Xuping Sun and Yongchao Yao

Biomedical Analysis, 2025, 2, 159
DOI: 10.1016/j.bioana.2025.11.004

Frontiers in Point-of-Care Testing: A Comprehensive Review

Hafsa Huma, Maryam Shabbir, Sung Eun Seo and Oh Seok Kwon

Appl. Sci. Converg. Technol., 2024, 33, 117
DOI: 10.5757/ASCT.2024.33.5.117

Bioinspired microfluidic chip for fluorescent detection of ultrafast and stable PCR

Long Huang, Bokai Luo, Jia Zhou and Lin Du

Eng. Res. Express, 2025, 7, 035573
DOI: 10.1088/2631-8695/ae024c

A Multi‐Volume Microfluidic Device for Automated and Wide Dynamic Range Digital LAMP Applications

Kaiyue Chen, Nan Rong, Chanqiong Zhang, Jian Xu, Hao He, Wenying Liu, Yinjia Xu, Yanzhao Wu, Qi Ouyang, Yunlong Zhou, Yaojun Yu, Wei Yang and Chunxiong Luo

Adv Materials Technologies, 2025, 10
DOI: 10.1002/admt.202401366

Droplet pair breakup in microfluidic expansion channel

Yihan Kuang, Tao Deng, Yingzhou Huang, Liyu Liu and Guo Chen

Applied Physics Letters, 2024, 124
DOI: 10.1063/5.0183413

Enhancing Sensitivity in Detecting Severe Fever With Thrombocytopenia Syndrome Virus: Development of a Reverse Transcription–Droplet Digital Polymerase Chain Reaction

Yuanyuan Zhang, Wen Tian, Shuai Zhang, Ling Lin, Chuan Song, Yuanni Liu, Yanli Xu, Ligang Zhang, Shuying Geng, Xin Li, Xi Wang, Zhihai Chen and Wei Zhang

The Journal of Infectious Diseases, 2025, 231, 512
DOI: 10.1093/infdis/jiae442

Microfluidics for the biological analysis of atmospheric ice-nucleating particles: Perspectives and challenges

Mark D. Tarn, Kirsty J. Shaw, Polly B. Foster, Jon S. West, Ian D. Johnston, Daniel K. McCluskey, Sally A. Peyman and Benjamin J. Murray

Biomicrofluidics, 2025, 19
DOI: 10.1063/5.0236911

A Microfluidic Chip for Single-Cell Capture Based on Stagnation Point Flow and Boundary Effects

Long Cheng, Xiao Lv, Wenchao Zhou, Huan Li, Qiushuang Yang, Xing Chen and Yihui Wu

Micromachines, 2024, 15, 456
DOI: 10.3390/mi15040456

Research progress and application exploration of typical microreactor technologies for health monitoring and disease Diagnosis/Treatment

Zhongjian Tan, Yun Zheng, Huanhuan Shi, Weizheng Xu, Xuanhao Jia, Zhengxian Dan, Jiacong Liao, Zhiying Dai and Chenyang Xu

Chemical Engineering Journal, 2024, 499, 155938
DOI: 10.1016/j.cej.2024.155938

A piezo pump based on ultrasonic resonance for supplying gas flow or pressure

Meng Wang, Runyu Liu, Duanyi Zhu, Yongxin Ma, Shuaiqi Cao, Conghui Wang, Xinbo Li, Xiaodong Sun and Guojun Liu

Sensors and Actuators B: Chemical, 2025, 424, 136931
DOI: 10.1016/j.snb.2024.136931

Molecular and Genetics-Based Systems for Tracing the Evolution and Exploring the Mechanisms of Human Norovirus Infections

Sheng-Chieh Lin, Geng-Hao Bai, Pei-Chun Lin, Chung-Yung Chen, Yi-Hsiang Hsu, Yuan-Chang Lee and Shih-Yen Chen

IJMS, 2023, 24, 9093
DOI: 10.3390/ijms24109093

A highly integrated digital PCR system with on-chip heating for accurate DNA quantitative analysis

Kang Peng, Zhihong Wu, Zhongxin Feng, Ruijun Deng, Xiangguo Ma, Beiyuan Fan, Haonan Liu, Zhuzhu Tang, Zijian Zhao and Yanzhao Li

Biosensors and Bioelectronics, 2024, 253, 116167
DOI: 10.1016/j.bios.2024.116167

Chimerism analysis by ABO blood group genotyping with digital droplet PCR

Takuya Naruto, Maiko Sagisaka, Mieko Ito, Akiko Hayashi, Naoyuki Miyagawa, Dai Keino, Tomoko Yokosuka, Fuminori Iwasaki, Hiroaki Goto and Masakatsu Yanagimachi

Int J Hematol, 2025, 121, 397
DOI: 10.1007/s12185-024-03898-7

A novel microfluidic chip-based digital PCR method for enhanced sensitivity in the early diagnosis of colorectal cancer via mSEPT9

Qunfang Huang, Zhen Xun, Junyu Lin, Rubing Xie, Chenggong Zhu, Long Wang, Hongyan Shang, Songhang Wu, Qishui Ou and Can Liu

Clinica Chimica Acta, 2024, 554, 117781
DOI: 10.1016/j.cca.2024.117781

Stationary Droplet Levitation via the Leidenfrost Effect: A Molecular Dynamics Study

Bin Xu, Lin Guo and Gongming Xin

Langmuir, 2025, 41, 23432
DOI: 10.1021/acs.langmuir.5c02255