Takumi Egashira, Hiroshi Kamizawa, Yujiro Hamada, Wataru Fushimoto, Hiroshi Y. Yoshikawa, Yuji Higaki, Atsushi Takahara, Takeshi Mori, Yoshiki Katayama and Akihiro Kishimura

Small, 2025
DOI: 10.1002/smll.202505876

Nucleic acid liquids

Gabrielle R Abraham, Aria S Chaderjian, Anna B N Nguyen, Sam Wilken and Omar A Saleh

Rep. Prog. Phys., 2024, 87, 066601
DOI: 10.1088/1361-6633/ad4662

Synthetic Biomolecular Condensates: Phase‐Separation Control, Cytomimetic Modelling and Emerging Biomedical Potential

Siyu Song, Tsvetomir Ivanov, Thao. P. Doan‐Nguyen, Lucas Caire da Silva, Jing Xie, Katharina Landfester and Shoupeng Cao

Angew Chem Int Ed, 2025, 64
DOI: 10.1002/anie.202418431

Synthetic nucleic-acid droplets: a bioprogramming platform for designer microliquids

Hirotake Udono, Tomoya Maruyama, Nathan N. Evangelista, Naoki Yoshida, Yuta Aizaki, Kei Goraku, Kanta Takagi, Ryoya Hasegawa and Masahiro Takinoue

Polym J, 2025, 57, 845
DOI: 10.1038/s41428-025-01050-8

Phase‐Separated Droplets Can Direct the Kinetics of Chemical Reactions Including Polymerization, Self‐Replication and Oscillating Networks

Iris B. A. Smokers, Brent S. Visser, Wojciech P. Lipiński, Karina K. Nakashima and Evan Spruijt

ChemSystemsChem, 2025, 7
DOI: 10.1002/syst.202400056

Construction of Somatostatin‐Based Multiphase “Core–Shell” Coacervates as Photodynamic Biomimetic Organelles

Wenyu Sun, Hongjie Xiong, Jiajia Yin, Wenyan Yao, Xiaohui Liu, Liu Liu, Xuemei Wang and Hui Jiang

Adv Healthcare Materials, 2025, 14
DOI: 10.1002/adhm.202403561

Autocatalytic Reaction between HCN and Cysteamine Produces Hydrophobic, Catalytic, Liquid Compartments

Alexander I. Novichkov, Yael Diskin-Posner, Linda J. W. Shimon, Gregory Leitus, Christoph Flamm and Sergey N. Semenov

J. Am. Chem. Soc., 2025
DOI: 10.1021/jacs.5c09581

Open questions on liquid–liquid phase separation

Evan Spruijt

Commun Chem, 2023, 6
DOI: 10.1038/s42004-023-00823-7

Cell‐Like Synthetic Supramolecular Soft Materials Realized in Multicomponent, Non‐/Out‐of‐Equilibrium Dynamic Systems

Ryou Kubota and Itaru Hamachi

Advanced Science, 2024, 11
DOI: 10.1002/advs.202306830

Small Amphiphile‐Based Coacervation

Xiao Xiao, Liyan Jia, Jianbin Huang, Yiyang Lin and Yan Qiao

Chemistry An Asian Journal, 2022, 17
DOI: 10.1002/asia.202200938

DNA Droplets: Intelligent, Dynamic Fluid

Hirotake Udono, Jing Gong, Yusuke Sato and Masahiro Takinoue

Advanced Biology, 2023, 7
DOI: 10.1002/adbi.202200180

Toward synthetic life—Emergence, growth, creation of offspring, decay, and rescue of fuel-dependent synthetic cells

Monika Wenisch, Yinqing Li, Marius G. Braun, Lukas Eylert, Fabian Späth, Simone M. Poprawa, Bernhard Rieger, Christopher V. Synatschke, Henrike Niederholtmeyer and Job Boekhoven

Chem, 2025, 102578
DOI: 10.1016/j.chempr.2025.102578

Optimization and Enhancement of the Peroxidase-like Activity of Hemin in Aqueous Solutions of Sodium Dodecylsulfate

Nemanja Cvjetan, Lukas D. Schuler, Takashi Ishikawa and Peter Walde

ACS Omega, 2023, 8, 42878
DOI: 10.1021/acsomega.3c05915

Self-growing protocell models in aqueous two-phase system induced by internal DNA replication reaction

Yoshihiro Minagawa, Moe Yabuta, Masayuki Su’etsugu and Hiroyuki Noji

Nat Commun, 2025, 16
DOI: 10.1038/s41467-025-56172-7

Probing the surface charge of condensates using microelectrophoresis

Merlijn H. I. van Haren, Brent S. Visser and Evan Spruijt

Nat Commun, 2024, 15
DOI: 10.1038/s41467-024-47885-2

Form Equals Function: Influence of Coacervate Architecture on Drug Delivery Applications

Chaeyoung Lim and Whitney C. Blocher McTigue

ACS Biomater. Sci. Eng., 2024, 10, 6766
DOI: 10.1021/acsbiomaterials.4c01105

Synthetic Biomolecular Condensates: Phase‐Separation Control, Cytomimetic Modelling and Emerging Biomedical Potential

Siyu Song, Tsvetomir Ivanov, Thao. P. Doan‐Nguyen, Lucas Caire da Silva, Jing Xie, Katharina Landfester and Shoupeng Cao

Angewandte Chemie, 2025, 137
DOI: 10.1002/ange.202418431

Selective Ion Binding and Uptake Shape the Microenvironment of Biomolecular Condensates

Iris B. A. Smokers, Enrico Lavagna, Rafael V. M. Freire, Matteo Paloni, Ilja K. Voets, Alessandro Barducci, Paul B. White, Mazdak Khajehpour and Evan Spruijt

J. Am. Chem. Soc., 2025, 147, 25692
DOI: 10.1021/jacs.5c07295

Heat‐Flow‐Driven Nonequilibria for Prebiotic Chemistry

Christof B. Mast

ChemSystemsChem, 2024, 6
DOI: 10.1002/syst.202400039

A roadmap toward the synthesis of life

Christine M.E. Kriebisch, Olga Bantysh, Lorena Baranda Pellejero, Andrea Belluati, Eva Bertosin, Kun Dai, Maria de Roy, Hailin Fu, Nicola Galvanetto, Julianne M. Gibbs, Samuel Santhosh Gomez, Gaetano Granatelli, Alessandra Griffo, Maria Guix, Cenk Onur Gurdap, Johannes Harth-Kitzerow, Ivar S. Haugerud, Gregor Häfner, Pranay Jaiswal, Sadaf Javed, Ashkan Karimi, Shuzo Kato, Brigitte A.K. Kriebisch, Sudarshana Laha, Pao-Wan Lee, Wojciech P. Lipinski, Thomas Matreux, Thomas C.T. Michaels, Erik Poppleton, Alexander Ruf, Annemiek D. Slootbeek, Iris B.A. Smokers, Héctor Soria-Carrera, Alessandro Sorrenti, Michele Stasi, Alisdair Stevenson, Advait Thatte, Mai Tran, Merlijn H.I. van Haren, Hidde D. Vuijk, Shelley F.J. Wickham, Pablo Zambrano, Katarzyna P. Adamala, Karen Alim, Ebbe Sloth Andersen, Claudia Bonfio, Dieter Braun, Erwin Frey, Ulrich Gerland, Wilhelm T.S. Huck, Frank Jülicher, Nadanai Laohakunakorn, L. Mahadavan, Sijbren Otto, James Saenz, Petra Schwille, Kerstin Göpfrich, Christoph A. Weber and Job Boekhoven

Chem, 2025, 11, 102399
DOI: 10.1016/j.chempr.2024.102399

Biphasic Coacervation Controlled by Kinetics as Studied by De Novo-Designed Peptides

Tianhao Ren and Dehai Liang

Langmuir, 2025, 41, 1756
DOI: 10.1021/acs.langmuir.4c04114

Selective peptide bond formation via side chain reactivity and self-assembly of abiotic phosphates

Arti Sharma, Kun Dai, Mahesh D. Pol, Ralf Thomann, Yi Thomann, Subhra Kanti Roy and Charalampos G. Pappas

Nat Commun, 2025, 16
DOI: 10.1038/s41467-025-56432-6

Coacervate‐Droplet Cased Synthetic Cells Regulated By Activated Carboxylic Acids (ACAs)

Matteo Valentini, Stefano Di Stefano and Job Boekhoven

ChemSystemsChem, 2025, 7
DOI: 10.1002/syst.202400083

Instructing Liquid‐Liquid Phase Separation Inside Membranous Protocells

Xintao Lv, Jiawei Liu, Peiyong Song, Li Zhao and Yiyang Lin

ChemSystemsChem, 2024, 6
DOI: 10.1002/syst.202300040

Compartmentalization as a ubiquitous feature of life: from origins of life to biomimetics

Souradeep Das, Gauri M. Patki, Vanthanaa Sridhar, Sahil Sunil Mulewar, Raya Roy, Udita Bandyopadhyay, Nishant Nitinidhi Kulshreshtha and Sudha Rajamani

Eur. Phys. J. Spec. Top., 2024, 233, 3153
DOI: 10.1140/epjs/s11734-024-01203-5

Multispecies autocatalytic RNA reaction networks in coacervates

Sandeep Ameta, Manoj Kumar, Nayan Chakraborty, Yoshiya J. Matsubara, Prashanth S, Dhanush Gandavadi and Shashi Thutupalli

Commun Chem, 2023, 6
DOI: 10.1038/s42004-023-00887-5

Resilient Membranized Coacervates Formed through Spontaneous Wrapping of Heat‐Destabilized Lipid Bilayers around Coacervate Droplets

Sadaf Javed and Evan Spruijt

Advanced Science, 2025, 12
DOI: 10.1002/advs.202412312

Coacervate-Based Materials: Fabrication, Structure, and Applications

Chong Wang and Luoran Shang

Chem. Mater., 2025, 37, 4944
DOI: 10.1021/acs.chemmater.5c01267

How Droplets Can Accelerate Reactions─Coacervate Protocells as Catalytic Microcompartments

Iris B. A. Smokers, Brent S. Visser, Annemiek D. Slootbeek, Wilhelm T. S. Huck and Evan Spruijt

Acc. Chem. Res., 2024, 57, 1885
DOI: 10.1021/acs.accounts.4c00114

Investigating coacervates as drug carriers using molecular dynamics

Yang Liu, Rongrong Zou, Yiwei Wang, Minghao Wang, Fan Fan, Yeqiang Zhou, Huixu Xie and Mingming Ding

Precision Medicine and Engineering, 2024, 1, 100012
DOI: 10.1016/j.preme.2024.100012

Peptide-Based Biomimetic Condensates via Liquid–Liquid Phase Separation as Biomedical Delivery Vehicles

Siyu Song, Tsvetomir Ivanov, Dandan Yuan, Jianqiang Wang, Lucas Caire da Silva, Jing Xie and Shoupeng Cao

Biomacromolecules, 2024, 25, 5468
DOI: 10.1021/acs.biomac.4c00814

Sticky Polyelectrolyte Shield for Enhancing Biological Half-Life of Growth Factors

Young Kim, Sungmi Jeon, Byulhana Kim, Yu Jin Jeong, Tae Hee Kim, Subin Jeong, Iljin Kim, Joomin Oh, Youngmee Jung, Kangwon Lee, Young Bin Choy, Sang Wha Kim and Justin J. Chung

ACS Appl. Mater. Interfaces, 2025, 17, 445
DOI: 10.1021/acsami.4c16261

Coacervate Vesicles as Adaptive Platforms for Synthetic Biology and Smart Materials

Francesco Vicentini, Federica Battistin and Pierangelo Gobbo

Chemistry A European J, 2025
DOI: 10.1002/chem.202502268

Recent advances in coacervation and underlying noncovalent molecular interaction mechanisms

Qiongyao Peng, Tao Wang, Diling Yang, Xuwen Peng, Hao Zhang and Hongbo Zeng

Progress in Polymer Science, 2024, 153, 101827
DOI: 10.1016/j.progpolymsci.2024.101827

Materials Inspired by Living Functions

Mauri A. Kostiainen, Arri Priimagi, Jaakko V. I. Timonen, Robin H. A. Ras, Maria Sammalkorpi, Merja Penttilä, Olli Ikkala and Markus B. Linder

Adv Funct Materials, 2024, 34
DOI: 10.1002/adfm.202402097

In Vitro Transcription–Translation in an Artificial Biomolecular Condensate

Ludo L. J. Schoenmakers, N. Amy Yewdall, Tiemei Lu, Alain A. M. André, Frank. H.T. Nelissen, Evan Spruijt and Wilhelm T. S. Huck

ACS Synth. Biol., 2023, 12, 2004
DOI: 10.1021/acssynbio.3c00069

Programmable Enzymatic Reaction Network in Artificial Cell‐Like Polymersomes

Hanjin Seo and Hyomin Lee

Advanced Science, 2024, 11
DOI: 10.1002/advs.202305760

Visualizing Formation and Dynamics of a Three-Dimensional Sponge-like Network of a Coacervate in Real Time

Ryou Kubota, Taro Hiroi, Yuriki Ikuta, Yuchong Liu and Itaru Hamachi

J. Am. Chem. Soc., 2023, 145, 18316
DOI: 10.1021/jacs.3c03793

Light‐Responsive Mononucleotide Coacervates

Edison Rafael Jimenez Granda, Hedi Karoui, Xavier Brilland, Jean‐Christophe Baret and Nicolas Martin

Chemistry A European J, 2025, 31
DOI: 10.1002/chem.202501109

Quantification of Biomolecular Condensate Volume Reveals Network Swelling and Dissolution Regimes during Phase Transition

Iris B. A. Smokers and Evan Spruijt

Biomacromolecules, 2025, 26, 363
DOI: 10.1021/acs.biomac.4c01201

Self-assembly of stabilized droplets from liquid–liquid phase separation for higher-order structures and functions

Mehwish Naz, Lin Zhang, Chong Chen, Shuo Yang, Hongjing Dou, Stephen Mann and Jianwei Li

Commun Chem, 2024, 7
DOI: 10.1038/s42004-024-01168-5

Chirality-Selected Coacervate by Chiral Gemini Surfactant

Lili Zhou, Jie Wang, Zhichen Xiong, Yaxun Fan and Yilin Wang

Langmuir, 2023, 39, 17488
DOI: 10.1021/acs.langmuir.3c02774

Effect of Phosphate on the Molecular Properties, Interactions, and Assembly of Engineered Spider Silk Proteins

Yin Yin, Alessandra Griffo, Adrián Gutiérrez Cruz, Hendrik Hähl, Karin Jacobs and Markus B. Linder

Biomacromolecules, 2024, 25, 3990
DOI: 10.1021/acs.biomac.4c00115

Toward Artificial Cell‐Mediated Tissue Engineering: A New Perspective

Yiğitcan Sümbelli, Alexander F. Mason and Jan C.M. van Hest

Advanced Biology, 2023, 7
DOI: 10.1002/adbi.202300149