Issue 13, 2023

Principles and applications of green fluorescent protein-based biosensors: a mini-review

Abstract

Green fluorescent proteins (GFPs) are useful and essential biomolecules that have revolutionized biosensor research. Researchers have extensively utilized GFPs for designing fluorescence biosensors due to their intrinsic fluorescence, high stability, and ability to undergo permutation or mutation. This review provides a concise summary of recent advancements in developing GFP-based biosensors. The construction approaches for GFP-based biosensors can be categorized into four types: (1) single GFP-based biosensors; (2) fluorescence resonance energy transfer-based biosensors; (3) GFP-based split biosensors; and (4) GFP chromophore analogy-based biosensors. We highlight the applications of these sensors in biomolecule detection and life sciences, while also sharing personal insights into the challenges associated with GFP-based biosensors and proposing future research directions.

Graphical abstract: Principles and applications of green fluorescent protein-based biosensors: a mini-review

Article information

Article type
Minireview
Submitted
28 feb 2023
Accepted
15 mai 2023
First published
17 mai 2023

Analyst, 2023,148, 2882-2891

Principles and applications of green fluorescent protein-based biosensors: a mini-review

F. Tian, G. Xu, S. Zhou, S. Chen and D. He, Analyst, 2023, 148, 2882 DOI: 10.1039/D3AN00320E

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