A TCF-based fluorescent probe to determine nitroreductase (NTR) activity for a broad-spectrum of bacterial species

Kai-Cheng Yan; Jordan E. Gardiner; Adam C. Sedgwick; Naing Thet; Rachel A. Heylen; Tony D. James; A. Toby A. Jenkins; Xiao-Peng He

doi:10.1039/D3CC00462G

A TCF-based fluorescent probe to determine nitroreductase (NTR) activity for a broad-spectrum of bacterial species†

Kai-Cheng Yan,

^ac Jordan E. Gardiner,

^a Adam C. Sedgwick,

^b Naing Thet,

^a Rachel A. Heylen,

^a Tony D. James,

*^ad A. Toby A. Jenkins

*^a and Xiao-Peng He

*^ce

Author affiliations

* Corresponding authors

^a Department of Chemistry, University of Bath, Bath, UK
E-mail: t.d.james@bath.ac.uk, a.t.a.jenkins@bath.ac.uk

^b Chemistry Research Laboratory, University of Oxford, Mansfield Road, UK

^c Key Laboratory for Advanced Materials and Joint International Research Laboratory of Precision Chemistry and Molecular Engineering, Feringa Nobel Prize Scientist Joint Research Center, School of Chemistry and Molecular Engineering, East China University of Science and Technology, 130 Meilong Rd., Shanghai 200237, China
E-mail: xphe@ecust.edu.cn

^d School of Chemistry and Chemical Engineering, Henan Normal University, Xinxiang 453007, China

^e National Center for Liver Cancer, The International Cooperation Laboratory on Signal Transduction, Eastern Hepatobiliary Surgery Hospital, the Second Military Medical University, Shanghai 200433, P. R. China

Abstract

A nitroreductase (NTR) responsive fluorescent probe with long wavelength fluorescence emission was used to determine the NTR activity of a selection of bacterial species under a range of different bacterial growth conditions ensuring applicability under multiple complex clinical environments, where sensitivity, reaction time, and the detection accuracy were suitable for planktonic cultures and biofilms.

This article is part of the themed collection: ChemComm Most Popular 2023 Articles

Supplementary files

Article information

DOI: https://doi.org/10.1039/D3CC00462G
Article type: Communication
Submitted: 01 ဖေ 2023
Accepted: 30 မေ 2023
First published: 15 ဇွန် 2023
This article is Open Access

Download Citation

Chem. Commun., 2023,59, 8278-8281

Permissions

Request permissions

A TCF-based fluorescent probe to determine nitroreductase (NTR) activity for a broad-spectrum of bacterial species

K. Yan, J. E. Gardiner, A. C. Sedgwick, N. Thet, R. A. Heylen, T. D. James, A. T. A. Jenkins and X. He, Chem. Commun., 2023, 59, 8278 DOI: 10.1039/D3CC00462G

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. You can use material from this article in other publications without requesting further permissions from the RSC, provided that the correct acknowledgement is given.

Chemical Communications

A TCF-based fluorescent probe to determine nitroreductase (NTR) activity for a broad-spectrum of bacterial species†

Abstract

Supplementary files

Article information

Download Citation

Permissions

A TCF-based fluorescent probe to determine nitroreductase (NTR) activity for a broad-spectrum of bacterial species

Social activity

Search articles by author

Spotlight

Advertisements