Issue 32, 2020

Microphysiological systems for the modeling of wound healing and evaluation of pro-healing therapies

Abstract

Wound healing is a multivariate process involving the coordinated response of numerous proteins and cell types. Accordingly, biomedical research has seen an increased adoption of the use of in vitro wound healing assays with complexity beyond that offered by traditional well-plate constructs. These microphysiological systems (MPS) seek to recapitulate one or more physiological features of the in vivo microenvironment, while retaining the analytical capacity of more reductionist assays. Design efforts to achieve relevant wound healing physiology include the use of dynamic perfusion over static culture, the incorporation of multiple cell types, the arrangement of cells in three dimensions, the addition of biomechanically and biochemically relevant hydrogels, and combinations thereof. This review provides a brief overview of the wound healing process and in vivo assays, and we critically review the current state of MPS and supporting technologies for modelling and studying wound healing. We distinguish between MPS that seek to inform a particular phase of wound healing, and constructs that have the potential to inform multiple phases of wound healing. This distinction is a product of whether analysis of a particular process is prioritized, or a particular physiology is prioritized, during design. Material selection is emphasized throughout, and relevant fabrication techniques discussed.

Graphical abstract: Microphysiological systems for the modeling of wound healing and evaluation of pro-healing therapies

Article information

Article type
Review Article
Submitted
28 ဖေ 2020
Accepted
24 ဇူ 2020
First published
29 ဇူ 2020

J. Mater. Chem. B, 2020,8, 7062-7075

Author version available

Microphysiological systems for the modeling of wound healing and evaluation of pro-healing therapies

H. E. Deal, A. C. Brown and M. A. Daniele, J. Mater. Chem. B, 2020, 8, 7062 DOI: 10.1039/D0TB00544D

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