Issue 5, 2020

Functionalization of NaGdF4 nanoparticles with a dibromomaleimide-terminated polymer for MR/optical imaging of thrombosis

Abstract

Herein, we report the development of a thrombosis-targeted molecular imaging probe with magnetic resonance (MR) and optical dual-modality capacity using dibromomaleimide (DBM)-bearing polymer-grafted NaGdF4 nanoparticles. The random copolymer of bisphosphonic ester (BPE)-P(OEGA-co-DBM) was first synthesized through reversible addition–fragmentation chain transfer (RAFT) copolymerization of oligo(ethylene glycol)methyl ether acrylate (OEGA) and DBM-based monomers using a BPE-terminated RAFT agent. The resulting polymers were subjected to deprotection with the formation of bisphosphonic acid (BPA) terminals, allowing for the attachment of the as-synthesized BPA-P(OEGA-co-DBM) chains onto the surface of paramagnetic NaGdF4 nanoparticles through the ligand exchange reaction. Azide moieties could be readily incorporated into the hybrid nanoparticles by the coupling reaction between the highly reactive DBM moieties and amine derivatives. Intriguingly, the coupling reaction was characterized by a unique fluorescence turn-on even in aqueous media, which subsequently enabled the fluorescence imaging applications of the resulting hybrid nanoparticle. Furthermore, a single-chain antibody (scFv), which is specifically used for the active conformation of the GPIIb/IIIa integrin, was successfully attached onto the nanoparticles by a strain-promoted copper-free “click” reaction, allowing the targeting of activated platelets in acute thrombosis. The hybrid nanoparticles prepared through this new surface functionalization protocol possessed not only high colloidal stability under physiological conditions but also potential MR/optical imaging capacity. The thrombosis targeting capacity of the hybrid nanoparticle-based probe was then demonstrated by exploiting DBM conjugation-induced fluorescence in living cells.

Graphical abstract: Functionalization of NaGdF4 nanoparticles with a dibromomaleimide-terminated polymer for MR/optical imaging of thrombosis

Supplementary files

Article information

Article type
Paper
Submitted
16 အောက် 2019
Accepted
10 ဒီ 2019
First published
10 ဒီ 2019

Polym. Chem., 2020,11, 1010-1017

Functionalization of NaGdF4 nanoparticles with a dibromomaleimide-terminated polymer for MR/optical imaging of thrombosis

Y. Li, F. Xin, J. Hu, S. Jagdale, T. P. Davis, C. E. Hagemeyer and R. Qiao, Polym. Chem., 2020, 11, 1010 DOI: 10.1039/C9PY01568J

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements