Determination of propranolol and hydrochlorothiazide by batch injection analysis with amperometric detection and capillary electrophoresis with capacitively coupled contactless conductivity detection

Abstract

We report two new electrochemical methods for the simultaneous determination of propranolol (PROP) and hydrochlorothiazide (HCT). One method is based on batch injection analysis with multiple pulse amperometric detection (BIA-MPA) and the other on capillary electrophoresis with capacitively coupled contactless conductivity detection (CE-C⁴D). The BIA-MPA procedure is highly precise (RSD < 2.1%, n = 10, for both PROP and HCT), fast (130 injections per h) and has low detection limits (0.17 and 1.9 μmol L⁻¹ for PROP and HCT, respectively). The proposed CE-C⁴D method allows the determination of PROP and HCT in less than 1 minute with high precision (RSD < 2.8%, n = 10, for both PROP and HCT) and low detection limits (30 and 10 μmol L⁻¹ for PROP and HCT, respectively). Both of the proposed methods were applied to the determination of PROP and HCT in pharmaceutical samples.