Perspective
Steric protection of near-infrared fluorescent dyes for enhanced bioimaging
Steric protection of a long-wavelength dye, by covalent steric appendage or non-covalent encapsulation, produces enhanced photostability, increased fluorescence brightness, decreased self-aggregation, and superior pharmacokinetic profile.
Dual-/Multi-Organelle-Targeted AIE Probes Associated with Oxidative Stress for Biomedical Applications
Proximity labeling expansion microscopy (PL-ExM) evaluates interactome labeling techniques
Proximity labeling expansion microscopy (PL-ExM) visualizes superresolution structures of interactome on widely accessible light microscopes, enabling the assessment of the precision and efficiency of proximity labeling techniques.
Paper
Study of transient absorption spectroscopy of a D–π–A structure aggregation-induced emission luminogen and its photodynamic therapy application
Transient absorption spectroscopy has revealed that AIEgens exhibit a more rapid and facile excited-state molecular motion in the solution state compared to the aggregate state, implying the predominance of nonradiative decay in the solution state.
Accepted Manuscript -
Paper
An Excimer Process Induced Turn-on Fluorescent Probe for Ultra-low Concentration Mercury Ions Detection
Clearly fluorescent delineating ER+ breast tumor incisal edge and identifying tiny metastatic tumor foci at high resolution
A turn-on estrogen receptor-positive (ER+) NIR fluorescent probe NB-TAM was prepared and successfully applied to clearly delineate the ER+ breast tumor incisal margin and identify very tiny metastatic tumor foci at high resolution.
Paper
Dopamine sensing by fluorescent carbon nanoparticles synthesized using artichoke extract
New fluorescent carbon nanoparticles, obtained using artichoke extract, have been used for the ultrasensitive (sub-nanomolar) and selective detection of dopamine in artificial saliva samples.
Paper
Development of a specific fluorescent probe to detect advanced glycation end products (AGEs)
A novel fluorescent probe, AGO, systematically detects advanced glycation end products involved in aging.
Structure–activity relationships of aniline-based squaraines for distinguishable staining and bright two-photon fluorescence bioimaging in plant cells
Regulation effects of three aniline-based squaraines were investigated. The first expression of distinct tissue labelling and high-resolution 3D reconstruction in the onion and plant cells induced by the subtle structure changes was further evaluated.
A small-molecule probe to decipher stress-induced ER microenvironments and ER-Golgi communication
A small molecule organic fluorophore has been developed to monitor the micropolarity inside endoplasmic reticulum in homeostatic and non-homeostatic conditions and visualize ER to Golgi transport.
Lysosomal-targeted fluorescent probe based pH regulating reactivity for tracking cysteine dynamics under oxidative stress
The ability to detect and visualize cellular events and associated biological analytes is essential for the understanding of their physiological and pathological functions.
About this collection
Fluorescence imaging is a predominant method employed in biological and biomedical research. This powerful technique utilises fluorescent probes that emit light upon excitation, enabling non-invasive and real-time observation of specific targets within the living system. It assists researchers in localizing specific proteins in tissue, visualizing subcellular structures, studying cell organization, monitoring the bio-microenvironment, and even conducting image-guided surgery. From fundamental biological studies to biomedical applications, fluorescence bioimaging continues to facilitate a more comprehensive understanding of the complex biological world and provide us with powerful tools for diagnosis or treatment.
This themed collection, guest edited by Dr Sijie Chen (The Chinese University of Hong Kong; Karolinska Institutet), Prof. Tony D. James (University of Bath), Dr Apurba L. Koner (IISER Bhopal) and Prof. Ben Zhong Tang (The Chinese University of Hong Kong, Shenzhen), aims to provide a platform for recent developments in this rapidly evolving field of fluorescence bioimaging with topics including, but not limited to, fluorescence bioimaging with novel probes, design or synthetic strategies of fluorescent probes, theoretical understanding of fluorescent probe properties and working mechanisms, and new insights into the materials chemistry of fluorescence bioimaging. We hope that readers find this themed collection informative and useful.