Issue 34, 2018

Real-time tracking of the autophagy process in living cells using plasmonically enhanced Raman spectroscopy of fucoidan-coated gold nanoparticles

Abstract

To date, a variety of biological assays such as immunostaining, western blotting, enzyme-linked immunosorbent assay (ELISA), and flow cytometry have been used to analyze and trace important biological events and therapies. In addition to these techniques, the application of microscopic analytical techniques such as matrix-assisted laser desorption/ionization-time of flight (MALDI-ToF) mass spectrometry and Raman spectroscopy is increasing, allowing information to be obtained at the molecular level. In this study, we have conducted real-time tracking of autophagy, a cellular process that has recently been attracting significant attention. To achieve this purpose, we performed Raman spectroscopy on human oral squamous carcinoma cells (HSC3) incubated with bioactive molecule-modified plasmonic gold nanoparticles. The bioactive molecule–nanoparticle complexes were synthesized using fucoidan, a biopolymer that induces autophagy. By using this platform, it was possible to trace the entire autophagic process successively from cell introduction to autophagic apoptosis. This fusion of nanocomposites and spectroscopic techniques is expected to enable more complex biological processes to be pursued at the molecular level in the future.

Graphical abstract: Real-time tracking of the autophagy process in living cells using plasmonically enhanced Raman spectroscopy of fucoidan-coated gold nanoparticles

Supplementary files

Article information

Article type
Paper
Submitted
29 мај 2018
Accepted
10 авг. 2018
First published
11 авг. 2018

J. Mater. Chem. B, 2018,6, 5460-5465

Real-time tracking of the autophagy process in living cells using plasmonically enhanced Raman spectroscopy of fucoidan-coated gold nanoparticles

H. Jang, K. Kang and M. A. El-Sayed, J. Mater. Chem. B, 2018, 6, 5460 DOI: 10.1039/C8TB01402G

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements