Issue 11, 2017

Microscopic evaluation of aqueous two-phase system emulsion characteristics enables rapid determination of critical polymer concentrations for solution micropatterning

Abstract

Aqueous two-phase systems have emerged as valuable tools for microscale analysis of cell growth and many other biotechnology applications. The most critical step in developing an aqueous two-phase system for a specific application is identifying the critical concentrations at which the polymer solutions phase-separate. Current techniques for determining these critical concentrations rely on laborious methods, highly specialized assays or computational methods that make this step difficult for non-specialists. To overcome these limitations, we present a simplified assay that uses only readily accessible laboratory instruments and consumables (e.g., multichannel micropipettes, 96-well plates and a simple compound microscope) to determine the critical concentrations of aqueous two-phase system-forming polymers. We demonstrate that formulations selected from phase diagrams that describe these critical concentrations can be applied for solution micropatterning of cells.

Graphical abstract: Microscopic evaluation of aqueous two-phase system emulsion characteristics enables rapid determination of critical polymer concentrations for solution micropatterning

Supplementary files

Article information

Article type
Paper
Submitted
13 фев. 2017
Accepted
03 мај 2017
First published
05 мај 2017

Analyst, 2017,142, 1938-1945

Microscopic evaluation of aqueous two-phase system emulsion characteristics enables rapid determination of critical polymer concentrations for solution micropatterning

M. Ruthven, K. R. Ko, R. Agarwal and J. P. Frampton, Analyst, 2017, 142, 1938 DOI: 10.1039/C7AN00255F

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